The Biocompatibility Research of Functional Schwann Cells Induced from Bone Mesenchymal Cells with Chitosan Conduit Membrane

Figures & data

Figure 1 The morphological difference of un-induced MSCs (A) and induced MSCs (B) under phase–contrast microscopy (100×).

Figure 2 Immunofluorescence localization of S-100 (A) and GFAP (B) in differentiated MSCs, and the corresponding nuclei counterstained with DAPI (C and D). The red region in cell body was the positive reaction region (100×).

Figure 3 Induced MSCs adhered to the chitosan membrane (100×).

Table 1. Absorption value of three groups (x ± s)

Group (n = 10)	5th day	7th day
Group A (control)	0.606 ± 0.083	0.629 ± 0.080
Group B (chitosan)	0.531 ± 0.070	0.600 ± 0.052
Group C (collagen + chitosan)	0.687 ± 0.086	0.693 ± 0.088

Three groups comparison at fifth day: P = 0.0008; comparison between two groups: PA, B = 0.042, PA, C = 0.048, PB, C = 0.0003; three groups comparison at seventh day: P = 0.03; comparison between two groups at seventh day: PA, B = 0.346, PA, C = 0.798, PB, C = 0.01.