A Method for Purification and Viral Inactivation of Human Placenta Hemoglobin

Figures & data

Figure 1 The concentration of PHb in different hemolytic time (n = 4).

Figure 2 Compare the influence of different deoxy methods on recovery and MetHb formation (n = 5).

Figure 3 Compare the influence of different reducing agents on recovery and MetHb formation.

Figure 4 Compare the influence of different stabilizers on recovery and MetHb formation (n = 5).

Figure 5 Compare the influence of different pH on recovery and MetHb formation (n = 5).

Figure 6 The percentage of MetHb formation during heating.

Figure 7 SDS-PAGE pattern of purified PHb and hemolysate Band 2, 4, purified PHb Band 1, 3, hemolysate.

Figure 8 Native-PAGE pattern of purified PHb and hemolysate Band 1, 3, 5, 7, purified PHb. Band 2, 4, 6, 8, hemolysate.

Figure 9 HPLC profile of hemolysate (a) and purified PHb (b).

Table 1 Purity of purified PHb

	Results (n = 5)
Methods of determinations	Purified PHb		Molecular weight
Native PAGE	Single band	99.52 ± 0.48% Pure
SDS-PAGE	Single band	99.90 ± 0.09% Pure	About 16 KD
HPLC	Single peak	99.89 ± 0.10% Pure	About 32 KD

Figure 10 Compare of spectrum characteristics of different Hb sample.

Table 2 Efficacy of exchange blood with Poly-PHb and Poly-AHb

	HCT (%)			PO2 (mmHg)
Sample	Before	After	The rate of EB (%)	Before	After	Survival (24 h)
Poly-PHb	45.5 ± 3.2	11.7 ± 3.3	74.6 ± 7.1	93.1 ± 13.9	131.4 ± 14.3	9/9
Poly-AHb	46.0 ± 1.5	13.6 ± 0.2	70.7 ± 10.2	87.3 ± 10.0	110.7 ± 32.1	9/9