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Original

Beneficial Effects of Freezing Rate Determined by Indirect Thermophysical Calculation on Cell Viability in Cryopreserved Tissues

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Pages 205-221 | Published online: 11 Jul 2009

Figures & data

Figure 1 The comparison between the experimental freezing rate and the calculated freezing rate using the direct thermophysical calculation for the HSV (A) and TEB (B).

Figure 1 The comparison between the experimental freezing rate and the calculated freezing rate using the direct thermophysical calculation for the HSV (A) and TEB (B).

Figure 2 The tcTs diagrams of the chamber temperature for the HSV (A) and the TEB (B).

Figure 2 The tc − Ts diagrams of the chamber temperature for the HSV (A) and the TEB (B).

Figure 3 The freezing rate (A), theoretically obtained but practically not existing, and the inverse calculation (B) of the chamber temperature for the HSV in a 180 ml freezing bag.

Figure 3 The freezing rate (A), theoretically obtained but practically not existing, and the inverse calculation (B) of the chamber temperature for the HSV in a 180 ml freezing bag.

Figure 4 The inverse calculations of the chamber temperature for PLGA with MC3T3-E1 cells (A) and CAp-AtCol+PLLA with NROs (B) in a 3.6 ml cryogenic vial.

Figure 4 The inverse calculations of the chamber temperature for PLGA with MC3T3-E1 cells (A) and CAp-AtCol+PLLA with NROs (B) in a 3.6 ml cryogenic vial.

Figure 5 The cellular viability of the cryopreserved HSV (A) and TEB (B), according to the freezing rates determined by direct or indirect thermophysical calculations.

Figure 5 The cellular viability of the cryopreserved HSV (A) and TEB (B), according to the freezing rates determined by direct or indirect thermophysical calculations.

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