Figures & data
Figure 1 The SEM photomicograph of Col-Chi-CS scaffold. A. The surface of scaffold. B. The cross-section of scaffold.
Figure 2 Scanning electron micrographs chondrocytes seeded in Col-Chi-CS scaffold. A. Cell aggregates grew and adhered to scaffold after 3 days. B. Cells are surround by synthesized matrix after 21 days cell-seeding.
Figure 3 H & E staining chondrocytes seeded in Col-Chi-CS matrix. A. Three days after seeding, × 100. B. Cells presented cartilage-specific lacunae in the superficial area 14 days after seeding, × 200. C. More homogeneous cartilage-like tissue were formed after 28 days seeding, × 100.
Figure 4 A. Chondrocyte-scaffold constructs implanted subcutaneously in the back of athymic nude mouse. Photographs of mouse immediately after implantation after 12 weeks. B. The H&E staining of Chondrocytes/Col-Chi-CS scaffold implanted in vivo for 4 weeks (× 100); C. The H&E staining of Chondrocytes/Col-Chi-CS scaffold implanted in vivo for 12 weeks (× 100). D. The H&E staining of Chondrocytes/Col-Chi-CS scaffold implanted in vivo for 12 weeks (× 400).
Figure 5 Toluidine blue staining of constructs confirmed chondorcytes abundance GAG after implanted in vivo for 12 weeks (× 100).
