855
Views
4
CrossRef citations to date
0
Altmetric
Original

Microbial Production of 7-Aminocepahlosporanic Acid and New Generation Cephalosporins (Cephalothin) by Different Processing Strategies

, &
Pages 345-358 | Published online: 11 Jul 2009

Figures & data

Table 1. CCD (central composite design) experimental plan for enzyme characterization

Figure 1 Growth profile and enzyme activity.

Figure 1 Growth profile and enzyme activity.

Table 2. CCD plan and measured response

Table 3. Result of regression analysis of the 23 factorial design

Table 4. Analysis of variance for characterization of cephalosporin-C acylase by Pseudomonas diminuta using central composite design criterion

Figure 2 Contour plot of enzyme activity vs temperature, pH.

Figure 2 Contour plot of enzyme activity vs temperature, pH.

Figure 3 Contour plot of enzyme activity vs temperature, agitation.

Figure 3 Contour plot of enzyme activity vs temperature, agitation.

Figure 4 Contour plot of enzyme activity vs agitation, pH.

Figure 4 Contour plot of enzyme activity vs agitation, pH.

Figure 5 Lineweaver-Burk plot for determination of Km and Vmax for cephalosporin-c acylase enzyme.

Figure 5 Lineweaver-Burk plot for determination of Km and Vmax for cephalosporin-c acylase enzyme.

Figure 6 Chromatogram of semisynthetic cephalosporin (Cephalothin).

Figure 6 Chromatogram of semisynthetic cephalosporin (Cephalothin).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.