Impact of Orally Administered Microcapsules on Gastrointestinal Microbial Flora: In-Vitro Investigation Using Computer Controlled Dynamic Human Gastrointestinal Model

Figures & data

Figure 1 Schematic representation of the dynamic simulated human gastro-intestinal (GI) model. Vessel 1: Stomach; Vessel 2: Small intestine; Vessel 3: Ascending colon; Vessel 4: Transverse colon; and Vessel 5: Descending colon.

Table 1. Effect of APPPA microcapsule on colon ascendance microbial population. The values have been shown in log colony-forming unit/ml (log CFU/ml)

	APPPA			Control
	0 h	6 h	12 h	0 h	6 h	12 h
Aerobes	3.16 ± 0.1	2.93 ± 0.01	2.94 ± 0.03	3.16 ± 0.1	2.86 ± 0.04	2.91 ± 0.11
Ecoli	3.28 ± 0.09	3.17 ± 0.09	3.4 ± 0.03	3.28 ± 0.09	2.83 ± 0.2	3.29 ± 0.18
Anaerobes	3.11 ± 0.05	2.99 ± 0.04	3.03 ± 0.01	3.11 ± 0.05	3.0 ± 0.03	2.97 ± 0.03
Lactobacilli	2.63 ± 0.04	2.56 ± 0.06	2.58 ± 0.6	2.63 ± 0.04	2.67 ± 0.1	2.55 ± 0.02

Table 2. Effect of APPPA microcapsules on colon transversum bacterial populations. The values have been shown in log colony-forming unit/ml (log CFU/ml)

	APPPA				Control
	0 h	6 h	12 h	24 h	0 h	6 h	12 h	24 h
Aerobes	3.4 ± 0.04	3.27 ± 0.02	3.3 ± 0.002	3.21 ± 0.02	3.4 ± 0.04	3.36 ± 0.01	3.37 ± 0.03	3.16 ± 0.1
Ecoli	3.28 ± 0.04	3.27 ± 0.06	3.24 ± 0.02	3.15 ± 0.04	3.28 ± 0.04	3.17 ± 0.06	3.31 ± 0.02	3.07 ± 0.01
Staph.	2.97 ± 0.05	2.49 ± 0.007	2.74 ± 0.02	2.6 ± 0.08	2.97 ± 0.05	2.79 ± 0.08	2.77 ± 0.08	2.54 ± 0.07
Anaerobes	3.41 ± 0.04	3.36 ± 0.01	3.29 ± 0.03	3.2 ± 0.05	3.41 ± 0.04	3.4 ± 0.05	3.4 ± 0.03	2.99 ± 0.01
Lactobacilli	3.29 ± 0.03	3.29 ± 0.02	3.22 ± 0.04	3.21 ± 0.06	3.29 ± 0.03	3.28 ± 0.08	3.38 ± 0.04	3.35 ± 0.03

Table 3. Impact of APPPA microcapsule on colon descendans bacterial populations. The values have been shown in log colony-forming unit/ml (log CFU/ml)

	APPPA			Control
	0 h	6 h	12 h	0 h	6 h	12 h
Aerobes	3.16 ± 0.09	2.97 ± 0.02	3.0 ± 0.08	3.16 ± 0.09	3.02 ± 0.05	3.06 ± 0.05
Ecoli	3.0 ± 0.13	3.03 ± 0.04	2.84 ± 0.02	3.0 ± 0.13	3.19 ± 0.001	2.86 ± 0.05
Staph.	3.11 ± 0.009	2.86 ± 0.1	2.75 ± 0.03	3.11 ± 0.009	3.09 ± 0.01	2.79 ± 0.01
Anaerobes	3.22 ± 0.12	3.26 ± 0.15	2.9 ± 0.02	3.22 ± 0.12	3.02 ± 0.19	2.88 ± 0.12
Lactobacilli	2.91 ± 0.01	2.89 ± 0.02	2.71 ± 0.05	2.91 ± 0.01	2.87 ± 0.005	2.88 ± 0.09

Figure 2 Impact of APPPA microcapsules on colon ascendance extracellular microbial enzymes.

Figure 3 Impact of APPPA microcapsule on colon transversum microbial extracellular enzymes.

Figure 4 Impact of APPPA microcapsule on colon descendans microbial extracellular enzyme activities.