Single Neural Progenitor Cells Derived from EGFP Expressing Mice is Useful After Spinal Cord Injury in Mice

Figures & data

Figure 1 Neurosphere isolated from hippocampi of E-GFP transgenic mice (E13.5). A: All cells could express GFP, which is exhibiting green color under the excitation light. B: Cells were immunoreactive staining for nestin. C: Merged photo. The bar is 100 uM.

Table 1. Percentage of the glia and neuron cells after 7-day differentiation of EGFP NSCs in vitro

Marker	Total number of green cells	Positive cells	Mean (%)±S.E
GFAP	1253	897	71.6±6.2
CNPase	1314	176	13.4±2.8
MAP2	1281	212	16.5±3.1

Figure 2 Representive photos for differentiation of EGFP-NSCs. A: GFAP positive cells. B: CNPase positive cells. C: MAP2 positive cells. D-F: Nuclei were stained with DAPI. G-I: Merged photos. The bar is 100 uM.

Figure 3 Percentage of the glia and neuron cells after 7-day differentiation of EGFP NSCs in vitro. A: Table 1: Percentage of the glia and neuron cells after 7-day differentiation of EGFP NSCs in vitro. B: The delineation of Table 1.

Figure 4 Differentiation of EGFP NSCs after transplantation into the leisured spinal cord in vivo. A: Transplantated EGFP positive cells. B: MAP2 positive cells. C: DAPI positive cells. D: Merged figure. Arrowheads denote double-labeled cells. The bar is 100 uM.