Figures & data
Figure 1 Analysis of the purity of purified porcine hemoglobin (A) and the molecular distribution of its glutaraldehyde-polymerized derivatives (B).
![Figure 1 Analysis of the purity of purified porcine hemoglobin (A) and the molecular distribution of its glutaraldehyde-polymerized derivatives (B).](/cms/asset/129db71f-7d63-4ce0-936a-2ab2a659ed4e/ianb19_a_258477_f0001_b.gif)
Figure 2 Silver-stained SDS-PAGE analysis of the purity of purified Hb and the molecular distribution of corresponding glutaraldehyde polymerized derivatives with 40 µg protein per lane (lane 1: pHb, lane 2: pPolyHb, lane 3: bHb, lane 4: bPolyHb, lane 5 :hHb and lane 6: hPolyHb).
![Figure 2 Silver-stained SDS-PAGE analysis of the purity of purified Hb and the molecular distribution of corresponding glutaraldehyde polymerized derivatives with 40 µg protein per lane (lane 1: pHb, lane 2: pPolyHb, lane 3: bHb, lane 4: bPolyHb, lane 5 :hHb and lane 6: hPolyHb).](/cms/asset/1a1c88db-767c-4361-a18d-d95797b71510/ianb19_a_258477_f0002_b.gif)
Table 1. Comparison of immunogenicity of different hemoglobin and their glutaraldehyde polymerized derivatives tested in mice
Figure 3 Western blotting analysis of cross-species binding reactions between a hemoglobin or its polymerized derivative and mouse polyclonal antibodies against a particular type of PolyHb.
![Figure 3 Western blotting analysis of cross-species binding reactions between a hemoglobin or its polymerized derivative and mouse polyclonal antibodies against a particular type of PolyHb.](/cms/asset/d9e3db14-fcc8-41d6-bdf6-6a54af346e3d/ianb19_a_258477_f0003_b.gif)
Table 2. Cross-species binding reaction (%) between glutaraldehyde polymerized hemoglobin and a particular ant-PolyHb polyclonal antibodies from rabbits or rats, as tested by a protein array method
Table 3. Amino acid sequence identity score among hemoglobin from different origins using ClustalW software for multiple alignment analysis