Effects of Pegylated Hamster Red Blood Cells on Microcirculation

Figures & data

Figure 1 (A) An un-anesthetized hamster rests comfortably in a custom plastic holder and the whole assembly is fitted under an intravital microscope. (B) 4X magnification of microvascular network in window. Arterioles (A), venules (V) and capillaries (C) are labeled accordingly. Note parallel arrangement of arteriole venule pair.

Table 1 Comparisons of hematocrit, hemoglobin, and plasma hemoglobin values in hamsters before and after injection of PEG-RBCs

		Treatment
Group	n	PEG (mM)	Size (kDa)	Hematocrit (%) pre-treatment	Hematocrit (%) 30 min	Hemoglobin (g/dl) pre-treatment	Hemoglobin (g/dl) 30 min	Free-Hb (g/dl) 30
1	4	control		49 ± 1	50 ± 2	15.2 ± 0.5	16.2 ± 0.6	0.3 ± 0.2
2	3	0.05	5	46 ± 4	47 ± 5	13.4 ± 1.5	14.4 ± 1.4	0.4 ± 0.3
3	2	0.5	5	50	53	15.5	16.7	0
4	2	1.1	5	51	52	16.4	16.6	0.4
5	3	2.2	5	49 ± 1	50 ± 2	15.4 ± 0.4	16.2 ± 0.6	0.1 ± 0
6	3	22	5	47 ± 3	46 ± 0	14.2 ± 1.1	14.4 ± 0.8	1.3 ± 0.2
7	5	0.05	20	48 ± 2	49 ± 2	14.6 ± 0.8	15.5 ± 1.0	0.2 ± 0.3
8	3	0.5	20	47 ± 5	51 ± 4	14.6 ± 2.1	15.8 ± 0.8	0.1 ± 0.1
9	4	5	20	47 ± 5	49 ± 4	14.7 ± 1.6	14.7 ± 1.6	0.5 ± 0.3

Note: The values are presented as mean ± standard deviation. n, animal numbers in a group; pre-treatment, before injection of PEG-RBCs; 30 min, test at 30 minutes after injection of PEG-RBCs; Free-Hb, plasma hemoglobin; control, injecting pure donor hamster RBCs. *, only mean values are presented since measurements were made in 2 animals.

Figure 2 The changes in venule diameter before, during, and after the injections of PEG-RBCs. The changes in venule diameter are presented by the ratios of venule diameter between post-and pre-treatments as function of time. No significant changes were recorded for the venule diameters (P > 0.05).

Figure 3 The changes in RBC velocity before, during, and after the injections of PEG-RBCs. The changes in RBC velocity are presented by the ratios of RBC velocities between post-and pre-treatments as function of time. The changes in RBC velocity were not significant (P > 0.05).

Table 2 Venule diameters in hamsters before, during, and after injection of PEG-RBCs

		Treatment
Group	n	PEG (mM)	Size (kDa)	Pre-treatment (μ m)	2 min (μ m)	5 min (μ m)	10 min (μ m)	20 min (μ m)	30 min (μ m)
1	4	control		31 ± 30	34 ± 33	33 ± 29	32 ± 28	32 ± 29	39 ± 33
2	3	0.05	5	30 ± 13	30 ± 14	28 ± 8	25 ± 9	32 ± 14	35 ± 15
3	2	0.5	5	36	36	43	34	36	47
4	2	1.1	5	19	18	19	19	18	20
5	3	2.2	5	28 ± 9	27 ± 12	29 ± 14	30 ± 12	30 ± 13	31 ± 12
6	3	22	5	41 ± 12	41 ± 11	41 ± 11	45 ± 16	45 ± 16	47 ± 16
7	5	0.05	20	21 ± 4	20 ± 3	22 ± 4	23 ± 4	22 ± 3	24 ± 5
8	3	0.5	20	59 ± 29	61 ± 31	54 ± 26	56 ± 27	54 ± 23	69 ± 4
9	5	5	20	22 ± 11	24 ± 14	24 ± 14	26 ± 16	30 ± 17	25 ± 15

Note: The diameters are presented as mean ± standard deviation. n, animal numbers in a group; pre-treatment, measurements were made before injection of PEG-RBCs; post-treatments including 2, 5, 10, 20, and 30 min after injection of PEG-RBCs; control, injecting pure donor hamster RBCs. *, only mean values are presented since measurements were made in 2 animals.

Figure 4 The changes in the interstitial partial oxygen pressure pO₂. The changes in pO₂ are presented by the ratios of pO₂ values between post-and pre-treatments as function of time. No significant changes were recorded for the pO₂ before, during, and after the injections of PEG-RBCs (P > 0.05).

Table 3 Values of interstitial partial oxygen pressure pO₂ in hamsters before, during, and after injection of PEG-RBCs

		Treatment
Group	n	PEG (mM)	Size (kDa)	pO2 (mm Hg) pre-treatment	pO2 (mm Hg) 2 min	pO2 (mm Hg) 5 min	pO2 (mm Hg) 10 min	pO2 (mm Hg) 20 min	pO2 (mm Hg) 30 min
1	4	control		19 ± 5	19 ± 5	19 ± 8	21 ± 12	21 ± 12	20 ± 10
2	3	0.05	5	43 ± 12	46 ± 10	48 ± 7	44 ± 5	47 ± 5	48 ± 2
3	2	0.5	5	24	24	22	20	21	20
4	2	1.1	5	27	25	21	23	22	19
5	3	2.2	5	31 ± 6	30 ± 5	29 ± 3	29 ± 2	29 ± 5	28 ± 1
6	3	22	5	25 ± 5	24 ± 5	25 ± 6	24 ± 5	24 ± 5	22 ± 7
7	5	0.05	20	26 ± 12	26 ± 11	24 ± 15	26 ± 18	25 ± 20	24 ± 20
8	3	0.5	20	28 ± 7	29 ± 8	32 ± 8	36 ± 14	42 ± 19	33 ± 5
9	4	5	20	23 ± 6	23 ± 6	23 ± 7	24 ± 5	24 ± 6	27 ± 10

Note: The values of pO₂ are presented as mean ± standard deviation. n, animal numbers in a group; pre-treatment, before injection of PEG-RBCs; post-treatments including 2, 5, 10, 20, and 30 min after injection of PEG-RBCs; control, injecting pure donor hamster RBCs. *, only mean values are presented since measurements were made in 2 animals.