Convection and Hemoglobin-Based Oxygen Carrier Enhanced Oxygen Transport in a Hepatic Hollow Fiber Bioreactor

Figures & data

Figure 1 Schematic of the convection enhanced hollow fiber bioreactor system utilized in the experimental study.

Figure 2 Oxygen consumption rate measured for each of the three experimentally operated hollow fiber bioreactor systems, at four different flow rates.

Figure 3 Averaged values of the ratio of lactate produced to glucose consumed for each of the three experimental hollow fiber bioreactor systems over the course of the entire experiment. Significant differences between the control case compared to the bRBC supplemented and convection enhanced cases are indicted by asterisks over the time point *(p < 0.025) and ** (p < 0.005).

Figure 4 Averaged values of accumulated albumin produced by each of the three experimental hollow fiber bioreactor systems over the course of the entire experiment. Error bars were determined from multiple assays of each sample.

Figure 5 Averaged values of total ammonia removal in each of the three experimental hollow fiber bioreactor systems over the course of the entire experiment. Error bars were determined from multiple assays of each sample.

Table 1 Key metabolic markers of metabolism, synthesis, and detoxification, for the last few days of the experimental study. All units are in μ g/(10⁶ cell · hr), except the unitless lactate to glucose ratio

Experimental system	Glucose consumption	Lactate production	Lactate glucose	Albumin synthesis	Ammonia removal
Control	0.363 ± 0.047	0.265 ± 0.036	1.46 ± 0.28	0.427 ± 0.35	1.10 ± 0.087
bRBC	0.783 ± 0.081	0.379 ± 0.027	0.967 ± 0.12	0.657 ± 0.17	2.50 ± 0.26
Convection	0.426 ± 0.11	0.196 ± 0.022	0.917 ± 0.26	0.809 ± 0.48	3.47 ± 0.25