Influence of PEG-conjugated Hemoglobin on Tumor Oxygenation and Response to Chemotherapy

Figures & data

Figure 1.  Representative photographs of the harvested tumors from each group and serial changes in pre-established tumor volume during each treatment in athymic mice.

Table 1. Effect of cisplatin, or cisplatin with PEG-Hb, or PEG-Hb alone on HeLa tumor cell growth in nude mice by intravenous administration (twice a week for four weeks)

	Increase of body weight (g)	Tumor weight (g)	Tumor Growth Inhibition (%)
Group 1	1.15±0.31	0.66±0.32	–
Group 2	−2.65±0.98	0.17±0.12	73.47
Group 3	−2.85±1.53	0.17±0.12	74.52
Group 4	−3.53±2.09	0.12±0.11	81.11
Group 5	0.93±0.16	0.48±0.36	20.54

Table 2. Pimonidazole binding scores in cervical cancer xenografts

	Group 1	Group 2	Group 3	Group 4	Group 5
Scores	2.81±0.86	2.93±0.79	1.93±0.71	0.82±0.55^▴	1.27±0.71

^▴P < 0.01 as compared with group1 and group 2.

Table 3. PCNA scores and apoptosis index in tumor sections

	Group 1	Group 2	Group 3	Group 4	Group 5
PCNA Scores	4.14±0.24	2.43±0.28	2.22±0.34	1.54±0.12*	4.04±0.21
Apoptosis Index (%)	4.12±1.24	32.5±3.28*	36.6±4.62*	45.4±8.72*	6.04±1.28

*P<0.01 as compared with Group 1 and Group 5. PCNA, Proliferating Cell Nuclear Antigen.