Biorheological Properties of Reconstructed Erythrocytes and its Function of Carrying-Releasing Oxygen

Figures & data

Figure 1.  Schematic drawing showing the system for micropipette aspiration.

Figure 2.  Dynamics analytical system of reconstruction erythrocyte carrying-releasing oxygen.

Figure 3A.  The electron scanning microscope patterns of reconstructed erythrocyte. 3040×.

Figure 3B.  The electron scanning microscope patterns of reconstructed erythrocyte with different size. 5500×.

Figure 3C.  The electron scanning microscope patterns of reconstructed erythrocytes. 3800×.

Figure 3D.  The electron scanning microscope patterns of reconstructed erythrocytes. 101×.

Figure 4.  Dynamical curve of oxygen saturation-dissociation of reconstitute erythrocyte.

Figure 5.  Dynamical curve of oxygen saturation-dissociation of Human blood.

Table 1. Biomechanical properties of reconstituted erythrocyte based on Hemispherical cap model (from Chien's theory)

	Elastic modulus(10^−3dyn/cm)	Viscous coefficient (10^−4dyn·s/cm)	S/V ratio
Reconstituted RBC	4.56∼7.89	0.18∼0.37	1.48∼1.53
Normal hRBC	1.12∼8.75	0.19∼0.45	1.39∼1.44

Table 2. Anti-hemorrhagic shock results of reconstituted erythrocyte blood

Test group	Name of group	Mean survival time (h)
A	Reconstructed erythrocytes blood	15.2±0.2
B	Wistar blood	17.5±0.3*
C	Wistar erythrocyte + saline	14.5±0.5
D	Wistar blood plasma	9.8±0.4**
E	Saline	3.8±0.6**
F	Liposome of Hb	8.0±0.3**
G	Hb + saline	6.0±0.5**
H	Anti-shock drug	13.0±0.2*

Compared with C group *: 0.01 < P<0.05, **: p < 0.01