Figures & data
Figure 1. DEAE cellulose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.
![Figure 1. DEAE cellulose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.](/cms/asset/176ea83f-2432-42f2-9004-a29a96e895ab/ianb19_a_374418_f0001_b.gif)
Figure 2. Q sepharose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.
![Figure 2. Q sepharose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.](/cms/asset/3bcd23cc-3673-4b96-9422-642e563d5218/ianb19_a_374418_f0002_b.gif)
Figure 3. Sephadex G-100 gel filtration chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.
![Figure 3. Sephadex G-100 gel filtration chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.](/cms/asset/79ca3871-0e68-4e25-9c34-bc78c13ffd71/ianb19_a_374418_f0003_b.gif)
Table 1. Purification steps for chicken erythrocyte GST
Figure 4. SDS-polyacrylamide gel electrophoresis of chicken erythrocyte GST (a) protein standards; (b) purified chicken erythrocyte GST.
![Figure 4. SDS-polyacrylamide gel electrophoresis of chicken erythrocyte GST (a) protein standards; (b) purified chicken erythrocyte GST.](/cms/asset/cf161b7a-76c4-485e-bb2d-19b8e2bc0113/ianb19_a_374418_f0004_b.gif)
Figure 6. pH stability of chicken erythrocyte GST. pH 7.5(… ♦ …), pH 7 (—○—), pH 8 (…□…), pH 9 (—Δ—), pH 6 (—▴—), pH 5 (— □___), pH 4 (…•…).
![Figure 6. pH stability of chicken erythrocyte GST. pH 7.5(… ♦ …), pH 7 (—○—), pH 8 (…□…), pH 9 (—Δ—), pH 6 (—▴—), pH 5 (— □___), pH 4 (…•…).](/cms/asset/ad139bb7-a3c8-4ff4-85dd-e6220c9803af/ianb19_a_374418_f0006_b.gif)
Figure 7. Heat stability of chicken erythrocyte GST. 10 °C (—•—), 20 °C (—□—), 30 °C (—▴—), 40 °C (—○—), 50 °C (— •—), 60 °C (… •…).
![Figure 7. Heat stability of chicken erythrocyte GST. 10 °C (—•—), 20 °C (—□—), 30 °C (—▴—), 40 °C (—○—), 50 °C (— •—), 60 °C (… •…).](/cms/asset/bbc7efcb-e217-45f9-a4a7-50302072e9c8/ianb19_a_374418_f0007_b.gif)
Figure 8. Effect of substrate (CDNB) concentration on chicken erythrocytes GST (Lineweaver-Burk plot).
![Figure 8. Effect of substrate (CDNB) concentration on chicken erythrocytes GST (Lineweaver-Burk plot).](/cms/asset/2f1b9e2c-884b-4fea-92ed-082810cedb08/ianb19_a_374418_f0008_b.gif)
Table 2. Effect of 2-mercaptoethanol and dithiothreitol on chicken erythrocyte GST after l hours incubation
Table 3. Effect of some chemicals on chicken erythrocyte GST after 1 hours incubation