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Original

Purification and Characterization of Glutathione-S-Transferase from Chicken Erythrocyte

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Pages 92-100 | Published online: 11 Jul 2009

Figures & data

Figure 1.  DEAE cellulose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.

Figure 1.  DEAE cellulose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.

Figure 2.  Q sepharose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.

Figure 2.  Q sepharose chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.

Figure 3.  Sephadex G-100 gel filtration chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.

Figure 3.  Sephadex G-100 gel filtration chromatography of chicken erythrocyte GST. (…•…) GST activity, (—o—) absorbance at 280 nm.

Table 1. Purification steps for chicken erythrocyte GST

Figure 4.  SDS-polyacrylamide gel electrophoresis of chicken erythrocyte GST (a) protein standards; (b) purified chicken erythrocyte GST.

Figure 4.  SDS-polyacrylamide gel electrophoresis of chicken erythrocyte GST (a) protein standards; (b) purified chicken erythrocyte GST.

Figure 5.  Optimum pH profile of chicken erythrocyte GST.

Figure 5.  Optimum pH profile of chicken erythrocyte GST.

Figure 6.  pH stability of chicken erythrocyte GST. pH 7.5(… ♦ …), pH 7 (—○—), pH 8 (…□…), pH 9 (—Δ—), pH 6 (—▴—), pH 5 (— □___), pH 4 (…•…).

Figure 6.  pH stability of chicken erythrocyte GST. pH 7.5(… ♦ …), pH 7 (—○—), pH 8 (…□…), pH 9 (—Δ—), pH 6 (—▴—), pH 5 (— □___), pH 4 (…•…).

Figure 7.  Heat stability of chicken erythrocyte GST. 10 °C (—•—), 20 °C (—□—), 30 °C (—▴—), 40 °C (—○—), 50 °C (— •—), 60 °C (… •…).

Figure 7.  Heat stability of chicken erythrocyte GST. 10 °C (—•—), 20 °C (—□—), 30 °C (—▴—), 40 °C (—○—), 50 °C (— •—), 60 °C (… •…).

Figure 8.  Effect of substrate (CDNB) concentration on chicken erythrocytes GST (Lineweaver-Burk plot).

Figure 8.  Effect of substrate (CDNB) concentration on chicken erythrocytes GST (Lineweaver-Burk plot).

Figure 9.  Effect of different diavalent cations on chicken erythrocyte GST activity.

Figure 9.  Effect of different diavalent cations on chicken erythrocyte GST activity.

Table 2. Effect of 2-mercaptoethanol and dithiothreitol on chicken erythrocyte GST after l hours incubation

Table 3. Effect of some chemicals on chicken erythrocyte GST after 1 hours incubation

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