Proteomic Diagnosis Models from Serum for Early Detection of Oral Squamous Cell Carcinoma

Figures & data

Figure 1.  Protein mass peak 4181 expressed significantly high in the serum of healthy individuals, while serum of OSCC patients presented a remarkably low protein mass peak at 4181 (P=0.0000000060).

Table 1. Cross-validation results of serum model of OSCC patients and healthy individuals (cases)

Groups	OSCC patients	Healthy volunteers	Total
OSCC patients	26	2	28
Healthy volunteers	2	30	32
Total	28	32	60

Note: the sensitivity and specificity were 93.75% (30/32) and 92.86% (26/28), respectively, and total accuracy was 93.33% (56/60).

Table 2. Seven selected discrepant serum protein mass peaks (m/z) comparison of OSCC patients with healthy individuals (x±SD)

Protein peaks	P value	Healthy volunteers	OSCC patients
4181.1701	0.0000000060	5981.53±2586.69	1626.68±1496.77
3163.9987	0.0000000370	4330.99±1584.81	1520.16±1570.63
4651.8335	0.0000001275	1930.55±555.61	934.18±519.63
5951.7588	0.0000002618	1147.50±304.64	1860.42±547.78
3979.0673	0.0000006163	1231.93±397.91	552.44±587.23
5985.1874	0.0000016396	1164.77±411.77	2083.43±774.80
4288.583	0.0000021997	4852.98±1541.91	2806.47±1147.62

Table 3. Five batches of sera and their proteomic diagnosis models of OSCC

Serum batches (cases)		Serum proteomic diagnosis models of OSCC
4	Normal 0 vs leukoplaque 1 (32:6)	4181		4651
3	leukoplaque 0 vs OSCC 1 (6:28)	4162				6886
5	Normal 0 vsOSCC 1 (28:32)	4181	4289	4652	5952, 5985	3164	3979
2	Normal 0 vs metastasis 1 (32:36)	4181			5952
1	OSCC 1 vs metastasis 0 (28:8)		4289		5661, 6195	4352	5072

Note: Italics represent high expressions in group 0, regular scripts represent high expression in group 1, black writings represent the consistency in the same row.

Figure 2.  Serum protein mass/charge wave peak 4289 was expressed highly in the normal volunteers group and low in the group of patients with OSCC tumor in batch 5, while it expressed highly in the group of patients with OSCC tumor and low in the group of metastasis patients with OSCC tumor in batch 1. (a) Serum batch 5, tumor 1 vs normal 0, m/z 4289. (b) Serum batch 1, tumor 1 vs metastasis 0, m/z 4289.