Correlation Between Single Nucleotide Polymorphism of Prothrombin Gene G20210 and Deep Vein Thrombosis after Total Joint Replacement in Chinese Patients

Figures & data

Figure 1.  Electrophoresis result of Prothrombin gene G20210 DNA amplification fragment in TJR patients. M: DNA Marker 1–10 patients number.

Figure 2.  Sequencing result of patient 39 who suffered DVT postoperatively. There was not a G-to-A mutation in the 20210^th position (276^th in the figure), but a G-to-T mutation in the 20208^th position (274^th in the figure) was found. AT of the patient was 117% preoperatively, and 89.8% postoperatively.

Table 1. Sequencing results of prothrombin gene G20210A of all patients

No.	Gender	Age (years old)	Surgery	Side	DVT	Mutation of prothrombin G20210A
1	F	57	TKR	Uni	yes	no
2	M	67	THR Revision	Uni	no	no
3	M	47	THR	Bi	no	no
4	M	37	THR	Uni	no	no
5	F	71	THR	Uni	yes	no
6	F	60	TKR	Bi	yes	no
7	F	66	TKR	Bi	yes	no
8	F	63	TKR	Uni	yes	no
9	F	76	THR	Uni	no	no
10	F	57	TKR	Bi	yes	no
11	F	67	TKR	Bi	Yes, proximal	no
12	F	70	TKR	Bi	no	no
13	F	66	TKR	Uni	yes	no
14	M	67	THR	Uni	no	no
15	F	72	THR	Uni	no	no
16	M	47	THR	Uni	no	no
17	F	51	THR	Uni	no	no
18	M	60	THR Revision	Uni	no	no
19	F	51	THR	Uni	no	no
20	M	45	THR	Bi	no	no
21	F	58	THR	Uni	Yes, previous	no
22	F	33	THR	Uni	no	no
23	F	59	THR	Uni	yes	no
24	M	76	TKR	Uni	no	no
25	F	74	TKR	Uni	yes	no
26	F	27	TKR	Uni	no	no
27	F	74	TKR	Uni	yes	no
28	F	23	THR	Uni	no	no
29	F	57	THR	Uni	yes	no
30	F	72	TKR	Bi	yes	no
31	F	47	THR	Bi	yes	no
32	F	58	TKR	Bi	yes	no
33	F	49	TKR	Uni	no	no
34	F	51	TKR	Uni	yes	no
35	F	68	TKR	Uni	Yes, previous	no
36	M	70	TKR	Uni	yes	no
37	M	40	THR	Bi	no	no
38	F	72	THR	Uni	no	no
39	F	60	TKR	Bi	yes	no*
40	F	66	TKR	Bi	Yes, previous	no
41	M	65	THR	Uni	no	no
42	F	43	THR	Uni	no	no
43	F	58	TKR	Uni	yes	no
44	F	56	THR	Uni	no	no
45	F	67	THR	Uni	no	no
46	F	70	TKR	Uni	yes	no
47	F	52	THR	Uni	no	no
48	F	64	TKR	Uni	yes	no
49	F	63	THR	Bi	yes	no
50	F	76	TKR	Bi	Yes, previous	no
51	M	75	TKR	Bi	no	no
52	F	60	THR	Uni	no	no
53	M	28	THR	Uni	no	no
54	F	66	TKR	Uni	Yes, proximal	no
55	F	50	THR	Uni	no	No

“previous” in the “DVT” column refers to preoperative DVT of lower limb(s) of the patient;

“*” refers to some other mutation of prothrombin gene at G20210 position, detailed in Figure 1.

Table 2. Statistic comparison of DVT group and non-DVT group after TJR

Item	DVT Group (n = 27	Non-DVT Group (n = 28)	t	x2	P
Age (years old)	63.67±7.14	53.18±16.58	3.066		0.004*
BMI (kg/ m2)	27.67±3.60	23.65±3.32	4.311		0.000*
Preoperative AT (%)	108.10±21.35	94.76±13.96	2.732		0.009*
Gender M	1 (37%)	12 (42.9%)
F	26 (96.3%)	16 (57.1%)		11.674	0.001*
Surgery TKR	21 (77.8%)	5 (17.9%)
THR	6 (22.2%)	23 (82.1%)		19.8	0.000*

*differentia between groups is significant.

Table 3. Genotype comparison of DVT group and non-DVT group after TJR

Genotype	DVT group (n = 27)		Non-DVT group (n = 28)
	Frequencies	Percentage	Frequencies	Percentage
Wild homozygote GG	27	100	28	100
Heterozygote GA	0	0	0	0
Mutational homozygote AA	0	0	0	0

Table 4. Allelic comparison of DVT group and non-DVT group after TJR

Allele	DVT group (n = 27)		Non-DVT group (n = 28)
	Frequencies	Percentage	Frequencies	Percentage
G	27	100	28	100
A	0	0	0	0