Comparisons of Collagen Gel and Collagen Matrix as Cell Growth Microenvironment: The Physical-chemical Properties

Figures & data

Table 1. Diameter of the dermises

	Gel dermis	Spongy dermis	t	P
1^std	60	60
4^thd	50.6±2.60768	57.2±0.83666	−5.38888	0.00065
8^thd	44.4±1.51658	56.2±1.30384	−13.1928	0.0001
12^thd	41.6±1.14018	48.8± 0.83666	−11.3842	0.0001

Figure 1.  The moisture content of the dermises.

Table 2. The denature temperature of the dermises (°C)

Specimen	1^std	4^thd	8^thd	12^thd
Gel dermis	82.4	82.7	89.2	92.1
Spongy dermis	112.3	110.1	106.5	98.4

Figure 2.  Snap intensity of the dermises (Kpa).

Figure 3.  SEM of the dermises (A: spongy dermis; B: gel dermis).

Figure 4.  H&E staining of the dermises (A: spongy dermis cultured for 4 days; B: spongy dermis cultured for 8 days; C: gel dermis cultured for 4 days; D: gel dermis cultured for 4 days).

Table 3. The stains of the FN and type I collagen of the two scaffolds *

Specimen	1 ^std	4 ^thd	8 ^thd	12 ^thd
Gel dermis	−	+	+	+ +
Spongy dermis	−	+	+ +	+ +

*+ positive; − negative

Figure 5.  Phase contrast micrographs of primary cultured fibroblasts.

Figure 6.  H&E staining of human skin.