Soluble Angiopoietin Receptor Tie-2 in Patients with Acute Myocardial Infarction and its Detection by Optical Protein-chip

Figures & data

Table 1. The inter-assay and intra-assay variation of ELISA and OPC-IE for detection of sTie-2

	Inter-assay variation			Intra-assay variation
ELISA
Sample	1	2	3	1	2	3
N	20	20	20	40	40	40
Mean concentration (ng/ml)	0.76	2.57	5.14	0.82	2.65	5.15
Standard deviation	0.04	0.11	0.26	0.07	0.17	0.27
CV (%)	4.6	4.4	5.0	8.3	6.5	5.2
OPC-IE
Sample	1	2	3	1	2	3
N	8	8	8	15	15	15
Mean concentration (ng/ml)	4.25	16.82	28.91	3.89	14.81	26.33
Standard deviation	0.46	2.05	3.85	0.34	1.45	1.84
CV (%)	10.8	12.2	13.3	8.7	9.8	7.0

Figure 1.  Optical protein-chip based on imaging ellipsometry for detection of sTie-2. (A) Image of the protein chip in gray scale format. (B) Three-dimensional image of the protein chip. Arrow area immobilized with antibody was used as reference. Other areas were added with different serum samples. After incubation with serum solution containing Tie-2, the thickness of the thin-layer increased (B).