Determination and Pharmacokinetic Study of Hydrocodone in Human Plasma by Liquid Chromatography Coupled with Tandem Mass Spectrometry

Figures & data

Figure 1.  The mass spectra of hydrocodone (A) and I.S. (B).

Figure 2.  The chromatograms of blank plasma (A), standard solution (B), and sample (C). 1: hydocodone, 2: I.S.

Table 1. Precision, accuracy and stabilities of hydrocodone in plasma (n = 5, x±sd)

	Nominal Conc.(ng·mL-1)	Found Conc.(ng·mL-1)	RSD(%)	RE(%)
Intra-day	1.0	0.97±0.01	1.18	−3.0
	10.0	10.52±0.33	3.15	5.2
	40.0	39.93±1.18	2.96	−1.8
Inter-day	1.0	0.97±0.05	5.55	−3.0
	10.0	10.56±0.32	3.05	5.6
	40.0	41.57±2.21	5.32	3.9
25°C,4h	1.0	0.96±0.04	4.27	−4.0
	10.0	9.78±0.53	5.44	−2.2
	40.0	39.75±1.24	3.11	−0.6
Freeze/thaw*	1.0	1.05±0.04	3.52	5.0
	10.0	10.65±0.52	4.83	6.5
	40.0	41.88±0.69	1.64	4.7
−20°C, 1day	1.0	1.02±0.03	2.50	2.0
	10.0	10.49±0.17	1.63	4.9
	40.0	41.11±0.52	1.27	2.8
−20°C, 7days	1.0	1.04±0.03	3.19	4.0
	10.0	9.82±0.53	5.40	−1.8
	40.0	41.21±1.55	3.76	3.0

*After three freeze/thaw cycles at −20°C.