Figures & data
Figure 1. The percent number of Jurkat cells alive, cultured in the presence of polyelectrolytes adsorbed on modified polypropylene support: poly-L-lysine (PLL) layer, bilayer of poly-L-lysine and poly-ethylenimine (PLL/PEI), or poly-L-lysine and poly-styrenosulfonate (PLL/PSS).
Figure 2. The percent number of Jurkat cells alive, after 5-day culture in the presence of polyelectrolytes adsorbed on modified polypropylene support: poly-allylamine (PAH) layer, or bilayer of poly-allylamine and poly-ethylenimine (PAH/PEI), or poly-allylamine and poly-styrenosulfonate (PAH/PSS).
Figure 3. The mean yield of nanocalsules of 5 milions HL-60 cells. PLL/PEI 1bl – cells covered with poly-L-lysine and polyethylenimine bilayer; PLL/PEI 3bl – cells covered three times with poly-L-lysine and polyethylenimine bilayer (cumulatively 6 layers); PLL/PSS 1bl–cells covered with poly-L-lysine and poly-styrenosulfonate bilayer; PLL/PSS 3 bl-cells covered three times with poly-L-lysine and poly-styrenosulfonate (cumulatively 6 layers); bl-bilayer.
Figure 4. The mean formazan production by nanoencapsulated and non-encapsulated (control) HL-60 cells. PLL/PEI 1bl – cells covered with poly-L-lysine and poly-ethylenimine; PLL/PEI 3bl – cells covered three times with poly-L-lysine and poly-ethylenimine bilayer (cumulatively 6 layers); PLL/PSS 1bl – cells covered with poly-L-lysine and poly-styrenosulfonate; PLL/PSS 3 bl-cells covered three times with poly-L-lysine and poly-styrenosulfonate (cumulatively 6 layers).
Figure 5. The mean formazan production by non-encapsulated (control) and nanoencapsulated rat islets of Langerhans during 48-hour culture. PAH – islets covered with poly-allylamine layer; PAH/PSS – islets covered with bilayer of poly-allylamine hydrochloride and poly-styrenosulfonate.
Figure 6. The mean formazan production by non-encapsulated (control) and nanoencapsulated rat islets of Langerhans during 8-day culture. PLL/PEI – islets covered three times with poly-L-lysine and poly-ethylenimine bilayer (cumulatively 6 layers).
Figure 7. Pancreatic rat islet coated with polyelectrolytes bilayer. SEM image (TM 1000, Hitachi, Japan), ×4000.
Figure 8. Light microscopic image of PE-coated rat pancreatic islets of Langerhans stained with dithizone.
Figure 9. HL-60 cell coated with triple bilayer of poly-L-lysine and poly-ethylenimine, deposited on filtration membrane. AFM image of surface (Nanoscope V, Veeco Instruments Inc., USA).
Figure 10. AFM image of microbeads coated with poly-L-lysine, showing an average layer thickness 17 nm. 3D representation of the height image.
Figure 11. AFM image of microbeads of 0,2 µm diameter. Left picture presents topography of microbeads. Right picture, which presents deflection error signal, is added to emphasize boundaries between microbeads.
