Electrochemical determination of hydrogen peroxide using a novel prussian blue–polythiophene–graphene oxide membrane-modified glassy carbon electrode

ABSTRACT

A polythiophene–graphene oxide compound membrane and Prussian blue were deposited sequentially on the surface of a glassy carbon electrode by cyclic voltammetry. Due to its excellent electrocatalysis and its analogy with peroxidase enzymes, Prussian blue has been widely used in amperometric biosensors. The polythiophene–graphene oxide compound membrane exhibited good electroconductibility and a large specific surface area. The fabricated Prussian blue/polythiophene/graphene oxide/glassy carbon electrode was characterized by transmission electron microscopy, scanning electron microscopy, and cyclic voltammetry. Under the optimal experimental conditions, the detection of hydrogen peroxide was studied by its amperometric current–time curve. Due to the presence of polythiophene–graphene oxide compound membrane and Prussian blue, the hydrogen peroxide sensor shows a linear calibration range of 1.0 × 10⁻⁶–1.0 × 10⁻⁴ mol L⁻¹, detection limits of 3.2 × 10⁻⁷ mol L⁻¹ at a signal-to-noise ratio of 3, and recoveries from 95.0 to 105.0%. The results show that the modified glassy carbon electrode has potential practical application for the determination of hydrogen peroxide based on its sensitivity and long-term stability.

KEYWORDS:

• Cyclic voltammetry
• polythiophene–graphene oxide membrane
• Prussian blue
• scanning electron microscopy
• transmission electron microscopy

Acknowledgments

This work was supported by the Shandong Province Natural Science Foundation of China (ZR2017MB049) and the University of Science and Technology Plan Projects in Shandong province (No. J17KA106 and J17KA108).