PRODUCTION AND CHARACTERIZATION OF L-FUCOSE DEHYDROGENASE FROM NEWLY ISOLATED Acinetobacter sp. STRAIN SA-134

Abstract

Microorganisms producing L-fucose dehydrogenase were screened from soil samples, and one of the isolated bacterial strains SA-134 was identified as Acinetobacter sp. by 16S rDNA gene analysis. The strain grew well utilizing L-fucose as a sole source of carbon, but all other monosaccharides tested such as D-glucose and D-arabinose did not support the growth of the strain in the absence of L-fucose. D-Arabinose inhibited the growth even in the culture medium containing L-fucose. Although the strain grew on some organic acids and amino acids such as citric acid and L-alanine as sole sources of carbon, the enzyme was produced only in the presence of L-fucose. The fucose dehydrogenase was purified to apparently homogeneity from the strain, and the native enzyme was a monomer of 25 kD. L-Fucose and D-arabinose were good substrates for the enzyme, but L-galactose was a poor substrate. The enzyme acted on both NAD⁺ and NADP⁺ in the similar manner.

Keywords:

• Acinetobacter
• enzyme purification
• enzyme properties
• fucose dehydrogenase

Notes

The strain SA-134 was cultivated in the synthetic medium with or without fucose adding indicated compounds at 0.5%. The medium with fucose in this Table corresponds to the synthetic medium containing 0.25% L-fucose described in the text. The cultivation was done at 30°C for 1 d. N.D. means not determined.