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Articles

Selective enrichment of milk fat globules using functionalized polyvinylidene fluoride membrane

, , , & ORCID Icon
Pages 18-27 | Published online: 27 Aug 2019
 

Abstract

We report on the development of a functionalized membrane-based technology for selective enrichment of milk fat globules from raw bovine milk. Functionalization was conducted by in situ polymerization of acrylic acid within a polyvinylidene fluoride membrane, followed by the electrostatic attachment of a cationic polymer to impart a net positive charge. The functionalized membrane-based technology enabled a one-step method of selective separation of globules directly from milk-based on size and charge. The presence of globules in the eluate was confirmed by fluorescence microscopy. Quantification of the extracted phospholipids from globules in the eluant revealed a significantly higher amount of polar lipids than the permeate. Our study describes a comprehensive analysis of selective enrichment of fat globules using a functionalized membrane and demonstrates the beneficial effect of extracted phospholipids from enriched fat globules.

Acknowledgments

The authors would like to thank the Institute Instrumentation Facility, Indian Institute of Technology Roorkee.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work is supported by Department of Biotechnology (DBT), Government of India; Grant No. BT/PR12721/AAQ/1/618/2015 to K.A. and Ministry of Human Resource Development to S.D.

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