A Simple and Quantitative Method for Analysis of Methoxyamine by Capillary Zone Electrophoresis

Abstract

Methoxyamine (MX) is a potential new chemotherapeutic agent. In structure, MX lacks a chromophore for spectrophotometric detection. Derivatization of MX with chromophoric 4‐(diethylamino)benzaldehyde (DEAB) was performed and the resultant MX derivative (DBMOH⁺) can be analyzed by capillary zone electrophoresis (CZE) with ultraviolet (UV) detection at 200 nm. The limit of detection (SD/S=3.3) of the method for MX was 2.50 µM with a 2 s injection at the pressure of 3.45 kPa. The linear calibration range for MX was 5.00–500 µM using N,N‐dimethyl‐p‐toluidine (DMPT) as the internal standard. The intra‐ and inter‐assay precision and accuracy of the method were ≤4% and within 98.4–104%, respectively. This method may be used for the quantitative determination of MX in pharmaceutical preparations.

Keywords:

• Methoxyamine
• Capillary zone electrophoresis