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Journal of Liquid Chromatography & Related Technologies Volume 29, 2006 - Issue 20
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Original Articles

Unified Reversed‐Phase Method for the Determination of Di (2‐Ethylhexyl) Phthalate and Its Major Metabolite, Mono (2‐Ethylhexyl) Phthalate, in Biological Samples

Debajit Sircar Department of Chemistry, Northeastern Illinois University, Chicago, Illinois, USA; Departments of Medicine and Biochemistry & Molecular Genetics, University of Illinois at Chicago, Chicago, IL, 60612, USA
,
Sargon J. Albazi Department of Chemistry, Northeastern Illinois University, Chicago, Illinois, USACorrespondence[email protected]
,
Yousef Atallah Department of Chemistry, Northeastern Illinois University, Chicago, Illinois, USA
&
William Pizzi Department of Psychology, Northeastern Illinois University, Chicago, Illinois, USA
Pages 2931-2942 | Received 20 Jul 2006, Accepted 30 Aug 2006, Published online: 07 Feb 2007
 
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Abstract

A UV detector at 235 nm and an Alltech Altima C18 column (150×4.6 mm and 5 micron) were used to develop a high performance liquid chromatographic method to determine Di (2‐ethylhexyl) phthalate [DEHP] and its metabolite mono (2‐ethylhexyl) phthalate [MEHP] in biological samples. A gradient time of 8 min and a gradient range of 60–100% acetonitrile (ACN) at pH 3.0, with a segmented flow rate gradient, were found to be optimum conditions. These conditions resulted in retention times of 4.2 and 7.1 min for MEHP and DEHP, respectively. The estimated limits of detection (LOD) and quantitation (LOQ) for DEHP were 1.37 and 4.8 µg/mL, respectively. For MEHP, LOD, and LOQ were 0.57 and 2.4 µg/mL, respectively. The developed method was applied to determine DEHP and its metabolite MEHP in blood plasma, liver, kidney, brain, and testis samples.

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