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Journal of Liquid Chromatography & Related Technologies Volume 30, 2007 - Issue 15: Thin Layer Chromatography
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Original Articles

TLC Analysis of a Corrinoid Compound from Dark Muscle of the Yellowfin Tuna (Thunnus albacares)

Michiko Nishioka Department of Health Science, Kochi Women's University, Kochi, Japan
,
Yuri Tanioka Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan
,
Emi Miyamoto Department of Health and Nutrition, Nagasaki International University, Sasebo, Japan
,
Toshiki Enomoto Department of Food Science, Ishikawa Prefectural University, Ishikawa, Japan
&
Fumio Watanabe Faculty of Agriculture, School of Agricultural, Biological and Environmental Sciences, Tottori University, Tottori, JapanCorrespondence[email protected]
Pages 2245-2252 | Received 04 Nov 2006, Accepted 29 Nov 2006, Published online: 28 Jun 2007
 
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Abstract

A significant amount of vitamin B12 (52.9±8.9 µg/100 g) was found in the dark muscle of the yellowfin tuna (Thunnus albacares), in comparison to that of the light muscle. A corrinoid compound was purified to homogeneity from the dark muscle and partially characterized. TLC and HPLC patterns of the purified corrinoid compound were identical to those of authentic vitamin B12. These results indicate that dark muscle of the yellowfin tuna would be an excellent source of vitamin B12 for humans and aminals.

Acknowledgment

This study was supported in part by a grant for Comprehensive Research on Cardiovascular Diseases from Ministry of Health, Labor and Welfare of Japan (to F. W.).

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