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Journal of Liquid Chromatography & Related Technologies Volume 33, 2009 - Issue 2
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Original Articles

APPLICATION OF TWO DIMENSIONAL THIN LAYER CHROMATOGRAPHY PATTERN COMPARISON FOR FINGERPRINTING THE ACTIVE COMPOUNDS IN THE LEAVES OF VITEX TRIFOLIA LINN POSSESSING ANTI-TRACHEOSPASMOLYTIC ACTIVITY

Alfi Khatib Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, Selangor Darul Ehsan, Malaysia; Department of Food Science and Technology, Bogor Agricultural University, Bogor, IndonesiaCorrespondence[email protected]
,
Arie C. Hoek Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, RA Leiden, The Netherlands
,
Selamat Jinap Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, Selangor Darul Ehsan, Malaysia
,
Md. Zaidul Islam Sarker Center of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, Selangor Darul Ehsan, Malaysia
,
Irwandi Jaswir Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia
&
Robert Verpoorte Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, RA Leiden, The Netherlands
Pages 214-224 | Published online: 29 Dec 2009
 
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Abstract

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase.

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