Abstract
Benzyl derivatives of hydroxystilbazole show biological activity appearing as antimicrobial and anticancer effect. Moreover, these derivatives show properties of liquid crystals and can form complexes. In this paper are presented optimum conditions of separation and determination of two isomers, derivatives of hydroxystilbazole by a technique of isotachophoresis. (E)-N-(benzyl)-4′-hydroxystilbazole bromide and (E)-N-(benzyl)-2′-hydroxystilbazole bromides were subjected to an optimization process. Lengths of analysis steps, electric current intensity and pH of electrolyte solutions and samples were changed during the optimization of conditions. The shortest time of analysis was obtained during determination of individual isomers. However, in the case of mixtures, the time of analysis was lengthened two times and was at about 420 seconds. It was proven by a process of trial and error that optimum pH for analyzed isomers are 3.7.
ACKNOWLEDGMENTS
The authors are cordially indebted to Prof. N. Erchak for making available the use of 4,4′-bis{1-(perhydroaminiomethyl)spirobi(1-sila-2,5-dioxacyclopentan-3-on)]at}, which was the main component of the terminating electrolyte.
Notes
1 - Staphylococcus aureus 209P FDA, 2 - Streptococcus faecalis ATCC 8040, 3 - Bacillus subtilis ATCC 1633, 4 - Escherichia coli PZHO 26B6, 5 - Klebsiella pneumoniae 231, 6 - Pseudomonas aeruginosa 5 R1, 7 - Candida albicanus PCM 1409 PZH, 8 - Microsporum gypseum K1, 9 - Aspergillus fumigatus C1.
a n = 5, the samples were analyzed twice.
b The sample was enriched with 1.5 mL of a solution containing 1 mg·mL−1of examined ion, n = 5.
c Correlation coefficient above 0.98.
d Calculated from the limit of identification and coefficients of the calibration curve.