COMPARISON OF NP-TLC AND RP-TLC WITH DENSITOMETRY TO QUANTITATIVE ANALYSIS OF IBUPROFEN IN PHARMACEUTICAL PREPARATIONS

Abstract

NP-TLC and RP-TLC techniques with densitometry have been established for the identification and the quantification of ibuprofen in selected pharmaceutical preparations, namely: Nurofen, Ibuprom, and Ibum. Analyses were performed on silica gel 60 F₂₅₄ plates using n-hexane-ethyl acetate-acetic acid, 15:5:0.7, v/v (NP-TLC) and on RP18 F_254s plates using methanol-water, 9:1, v/v (RP-TLC). UV densitometry was performed at 200 and 224 nm for NP-TLC and RP-TLC analysis, respectively. NP-TLC and RP-TLC techniques with the densitometry and the spectrodensitometry applied to the investigation and the determination of ibuprofen in pharmaceutical preparations are selective, precise, and accurate. The techniques also realize the criterion of the linearity in the required range of ibuprofen concentrations.

Keywords:

• densitometry
• NP-TLC
• pharmaceutical analysis
• quantitative analysis
• RP-TLC
• spectrodensitometry

ACKNOWLEDGMENT

This research was financed by the Ministry of Science and Higher Education by resources reserved for science in the years 2005–2008 as research project No. 3T09A 155 29.

Notes

^a SD – standard deviation.

RSD – relative standard deviation.