Abstract
High speed countercurrent chromatography was applied to the separation of racemic ofloxacin using L-(+)-tartaric acid as chiral selectors. The two-phase system composed of ethyl acetate/methanol/water = 10:1:9 was chosen. The upper phase contained 200 mmol/L of chiral selector. The maximum amount of sample in a one-time injection can be up to 20 mg. The enantiomers separated were identified by HPCE, which confirmed that this method was very useful for the chiral preparative separation.
ACKNOWLEDGMENT
The work is supported by National Natural Science Foundation and Yunnan Province's Natural Science Foundation of China.