A MICELLAR LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF CARBARYL AND 1-NAPHTHOL IN BIOLOGICAL SAMPLES

Abstract

Micellar liquid chromatography (MLC) is a useful technique for the determination of compounds in biological samples. Here, a sensitive procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite, 1-naphthol, using a C18 column (150 mm × 4.6 mm) and fluorescence detection. After performing optimization studies with an interpretative strategy, the selected mobile phase was sodium dodecyl sulfate (SDS) 0.15 M – 6% (v/v) pentanol - NaH₂PO₄ 0.01 M buffered at pH 3. The validation studies include linearity (r² > 0.9999), limits of detection and quantification, (0.0325 and 0.1 µM for carbaryl; 0.125 and 0.350 µM for 1-naphthol, respectively), intra- and inter-day precisions below 4%, and robustness parameters below 5%, all of which are adequate for the quantification of these two compounds in urine and serum samples to be directly injected into the chromatographic system.

Keywords:

• 1-naphthol
• biological samples
• carbaryl
• direct injection
• fluorescence
• micellar liquid chromatography

ACKNOWLEDGMENTS

Sandeep K. Mourya thanks U.G.C. (GoI) in New Delhi for providing J.R.F. Maria Rambla-Alegre thanks the M.E.C. for its research fellow. Dr. Devasish Bose thanks Bancaixa for providing the visiting grant. This study was part of Project EMR-15(1)/2005/C-06, funded by Directorate of Forensic Science (MHA, GoI) at the Dr. H.S. Gour University, Sagar. Project CTQ2007-64473/BQU was funded by the MEC.

Notes

c₁, c₂, c₃, and c₄ were 0.5, 1.25, 2.5, and 3.75 µM, respectively, for carbaryl and 1-naphthol.