PREPARATION AND CHARACTERIZATION OF BONDED-PHASES OF NANO-BASKETS BEARING SULFONYL-CARBOXAMIDE

Abstract

A new sensitive method for determination of clenbuterol, as a nutrient repartitioning agent, in livestock is presented to protect the consumers. Four derivatives of p-tert-calix[4]arene bearing di-[N-(X)sulfonyl carboxamide] and di-(1-propoxy) in para-cone conformation were synthesized as nano-baskets and were used to prepare bended phases of HPLC–UV. The new synthesized bonded-phases were characterized and optimized, the bonding interactions of solute and stationary phases were examined and the main interactions were reported. The results revealed that for the best bonded-phase, the LOD and LOQ were 0.01 and 0.05 µg/mL, respectively. Supplemental materials are available for this article. Go to publisher's online edition of Journal of Liquid Chromatography & Related Technologies to view the free supplemental file.

Keywords:

• bonded-phase
• calixarene
• clenbuterol
• livestock
• nano-basket
• repartitioning agent

ACKNOWLEDGMENT

This work was supported by Islamic Azad University (Shahreza branch) and Iran Nanotechnology Initiative Council.