ISOLATION AND PURIFICATION OF PAEONIFLORIN AND ALBIFLORIN FROM RADIX PAEONIAE RUBRA BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

Abstract

Paeoniflorin and albiflorin were isolated and purified from Radix Paeoniae Rubra by high-speed counter-current chromatography with a solvent system of ethyl acetate-n-butanol-water (3:2.5:5, v/v/v) in one step. From 100 mg of the n-butanol extract of Radix Paeoniae Rubra, 10 mg of paeoniflorin and 4.3 mg of albiflorin were obtained with purity of 98.75% and 95.03%, as determined by HPLC. Their structures were identified by MS, UV, and NMR analysis. In this study, a rapid method for isolation and purification of the two major compounds from Radix Paeoniae Rubra crude extract was established.

Keywords:

• albiflorin
• HSCCC
• isolation
• paeoniflorin
• purification
• Radix Paeoniae Rubra

ACKNOWLEDGMENT

This work was supported by the International Scientific and Technological Cooperation Program of China (No: 2009DFA31230) and the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No: 2010B090400533).

Notes

K, solute concentration in the upper mobile phase divided by that in the lower stationary phase.