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Abstract
Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-fucoxanthin from showed one major peak with a retention time of 7.8 min.
ACKNOWLEDGMENT
This work was financially supported by a research grant (IIUM RMGS 09-05) from the Research Management Center, International Islamic University Malaysia (IIUM). We thankfully acknowledge Assoc. Prof. Masashi Hosokawa, M.K. Widjaja-Adhi Airanthi, and Masatoshi Nomura from the Laboratory of Biofunctional Material Chemistry, Hokkaido University, Hakodate, Hokkaido, Japan, for their excellent technical support. Thanks to Hendri (Andi) Ahmad Muzamil from the Natural Product Research Laboratory, Faculty of Science, IIUM Kuantan, Pahang, Malaysia, for his technical support.
Notes
The fucoxanthin was detected at 450 nm. Fucoxanthin standard (>99% purity established by HPLC) was prepared from wakame (Undaria pinnatifida).
*The experiment has been done triplicate (n = 3).
OCC: Open Column Chromatography; RP-HPLC: Reverse Phase-HPLC.
Note. The Spots were not Observed under UV with λ = 254 and 366 nm and without spraying. The Rf values were directly counting from TLC plates.n-Hexane/acetone ratio 9/1 is not determined.