DETERMINATION OF ZEARALENONE BY THE CAPILLARY ZONE ELECTROPHORESIS-UV DETECTION AND ITS APPLICATION TO POULTRY FEED AND CEREALS

Abstract

A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20 mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15 kV, injecting 10 s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254 nm. Zearalanon and internal standard appeared at 5.46 min (RSD% 0.20) and 6.35 min (RSD% 0.18), respectively. The repeatability results are in the range of 0.01–1.58 for inter-day. The linearity was investigated in the range of 5.20 × 10⁻⁷ M to 7.86 × 10⁻⁵ M ZEA and it was linear fitting to the equation of [rPN = 244803.8 C(M) −0.0348; r = 0.9999]. The LOD and LOQ were calculated to be 2.70 × 10⁻⁸ M (8.25 µg/L) and 8.17 × 10⁻⁸ M (25 µg/L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.

Keywords:

• capillary electrophoresis
• grain
• maize flour
• mycotoxin
• poultry feed
• zearalenone

ACKNOWLEDGMENT

The authors are grateful to Eskişehir Osmangazi University Scientific Research Projects (Project No. 200719037) for their financial support for this work.

Notes

^a ZEA amount corrected (µg/L) according to the recovery results by using the equation: Found ZEA (µg/L) = 0.6892 Added ZEA (µg/L) + 176.37.

^b RSD(%); relative standard deviation.