DETERMINATION OF AFLATOXIN B1 AND B2 IN PEANUT AND PEANUT OIL USING CLOUD POINT EXTRACTION FOLLOWED BY ULTRA-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

A new approach for ultra-high-performance liquid chromatography with fluorescence detection (UHPLC-FLD) determination of aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) in peanut and peanut oil by cloud point extraction (CPE) has been developed. The nonionic surfactant Triton X-114 was chosen to extract the target aflatoxin. The different parameters affecting the extraction of target analytes such as pH, concentration of surfactant, and temperature were investigated and optimized. Linear calibration curves were obtained in the range of 1.0–1000.0 ng/mL under optimum conditions. Recoveries ranged of 90.17–96.25% and the relative standard deviations (RSD) were 1.13–4.12% (n = 5). All correlation coefficients of the calibration curves were higher than 0.999. The intra-day precision for 1.13–4.12% and inter-day precision for 1.30–5.12%, and the limits of detections (LODs) were 0.40 ng/mL and 0.20 ng/mL for AFB1 and AFB2, respectively. And, the concentration factor was 20. The method was applied to the simultaneous determination of AFB1 and AFB2 in peanut and peanut oil.

Keywords:

• aflatoxin
• cloud point extraction
• enrichment
• FLD
• surfactant
• UHPLC

ACKNOWLEDGMENT

Financial support by the College Students' National Innovation Fund (No.101067405), which was provided by Kunming University of Science and Technology, is gratefully acknowledged. Additionally, this study was supported by the Medical Neurobiology Key Laboratory of Kunming University of Science and Technology, Basic and Applied Research Project in Yunnan province (2008ZC082M) and the authors are grateful for this support.

Notes

^a The concentration factor was the volume ratio of Triton X-114 and micelle phase.

^a Spiked peanut oil sample.

^b Spiked peanut sample.

^a x is the concentration (ng/mL) and y is the peak area.

^a Spiked peanut oil sample.

^b Spiked peanut sample.

^c Spiked by 1 ng/g.

^d Spiked by 10 ng/g.

^e Spiked by 20 ng/g.

^a Spiked peanut oil sample.

^b Spiked peanut sample.

^c Spiked by 1 ng/g.

^d Spiked by 10 ng/g.

^e Spiked by 20 ng/g.