SIMULTANEOUS DETERMINATION OF TEN STILBENES IN THE SEEDS OF PAEONIA SPECIES USING HPLC-DAD

Abstract

A rapid, sensitive, and accurate HPLC-DAD method was developed and validated for simultaneous determination of 10 stilbenes, which include suffruticosol A, suffruticosol B, suffruticosol C, resveratrol (E)-form, cis-ϵ-viniferin, trans-ϵ-viniferin, cis-suffruticosol D, cis-gnetin H, trans-suffruticosol D and gnetin H, in 44 plant samples from 9 different Paeonia species. In this optimized method, separations were carried out on an YMC-pack ODS-A column with water and methanol as mobile phase. The flow rate was set at 1.0 mL/min and the detector wavelength was set at 230 nm. The results showed that all the 10 analytes showed good linearity (r² > 0.9994). The recoveries, measured at three concentration levels, varied from 97.19% to 102.89%. This method was also validated with respect to precision, repeatability, stability, and successfully applied to simultaneous determination of 10 stilbenes in 44 batches of peony seeds from 9 Paeonia species collected from different areas.

Keywords:

• chemical profiling
• HPLC–DAD
• Paeonia
• peony seed
• quantification
• stilbenes

ACKNOWLEDGMENT

The authors are grateful to Prof. Lian-ying Wang and Fu Wang from the College of Landscape Architecture, Beijing Forestry University, and Mrs. De-zhong Chen from Peace Moutan Garden of Lanzhou for providing peony seeds. This work was supported by the National Basic Research Program of China (973 program) (Grant No. 2006CB504701) and Major Research Plan of the National Natural Science Foundation of China (Grant No. 30530860).

Notes

^a Content = mean ±SD (n = 3).

^b Samples having same sources were collected from different cultivated farms in the same regions.

^c The denotations from 1 to 10 are the corresponding chemicals as listed in Fig. 1.

^d ‘tr.': below the LOQ;.

^e ‘nd.': below the LOD.

^a The compound code of each analyte refers to Figure 1.

^b y, peak area; x, concentration of compound (μg/μL).

^a The compound code of each analyte refers to Figure 1.

^b Recovery (%) = (detected amount − original amount)/spiked amount × 100.

^c RSD (%) = (SD/mean) × 100.