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Abstract
A novel approach to single-run determination of 25 free amino acids, L-carnosine, 4 nitrogen bases (purine and pyrimidine), and 5 nucleosides in unpurified biological samples is reported. The analytes were extracted from the sample, derivatized with dansyl chloride, and analyzed using RP-HPLC-DAD-ESI-MS. The reported method features high sensitivity (LOQs in 5–10 ng mL−1 range), wide linearity range with r > 0.94, and high precision (intra-day RSD within the 0.1–4.2% range). Analyte average recovery coefficient was in the 70.1–111.3% range. The method was used to determine levels of free amino acids, L-carnosine, nitrogen bases, and nucleosides in beef (strip loin). Evolution of concentrations of the studied compounds during meat storage processes (vacuum packing, cold storage) was also investigated.
ACKNOWLEDGMENT
Research was conducted in the Project Optimizing of Beef Production in Poland According to “From Fork to Farm” Strategy co-financed by the European Regional Development Fund under the Innovative Economy Operational Program (Contract No. UDA-POIG.01.03.01-00-204/ 09-03).
Notes
sd = standard deviation.
Color versions of one or more of the figures in the article can be found online at www.tandfonline.com/ljlc.