![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
Quantification of polycyclic aromatic hydrocarbons (PAHs) in lung tissue samples for the estimation of clinical impact was carried out.
Methods and Materials: A high pressure liquid chromatography system (HPLC) with ultraviolet-visible diode array detector (DAD) and fluorescence (FLD) detection was used. An automated solvent extraction (ASE) procedure with sample clean-up was employed for the extraction of PAHs from the lung tissue samples. The chromatographic method allowed identification of a total number of 16 PAHs: naphtalene, acenaphthene, fluorene, phenanthrene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(ghi)perylene, and indeno(1,2,3-cd)pyrene using fluorescence detection, whereas acenaphtylene was identified using a diode array detector (DAD).
Results: The linearity domain for the method was between 5 pg/µL and 400 pg/µL for each compound. The quantification limits were between 0.5 pg/µL and 20 pg/µL. Automated solvent extraction was used for obtaining recoveries between 82–120%.
ACKNOWLEDGMENT
The authors thank Patricia Fiterman, executive manager at Fiterman Pharma Iasi, Romania for the opportunity to extend our study in the company's Quality Control Department where we found all necessary techniques for fully developing of the project.