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Journal of Liquid Chromatography & Related Technologies Volume 37, 2014 - Issue 18
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Original Articles

SEPARATION OF THREE BIOACTIVE ISOMERS FROM BIDENS PILOSA BY COUNTERCURRENT CHROMATOGRAPHY

Li Ping College of Life and Environmental Sciences, Minzu University of China, Beijing, China
,
Wang Yeling College of Life and Environmental Sciences, Minzu University of China, Beijing, China
,
Guo Zhiyong College of Life and Environmental Sciences, Minzu University of China, Beijing, China
,
Shibiao Wu Department of Biological Sciences, Lehman College, and The Graduate Center, City University of New York, New York, USA
,
Edward J. Kennelly College of Life and Environmental Sciences, Minzu University of China, Beijing, China; Department of Biological Sciences, Lehman College, and The Graduate Center, City University of New York, New York, USA
,
Wang Jianjun College of Life and Environmental Sciences, Minzu University of China, Beijing, China
,
Tang Li College of Life and Environmental Sciences, Minzu University of China, Beijing, China
&
Long Chunlin College of Life and Environmental Sciences, Minzu University of China, Beijing, China; Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaCorrespondence[email protected]
 show all
Pages 2598-2609 | Published online: 20 May 2014
 
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Abstract

Under LC-TOF-MS fraction-guided analysis, the n-butanol was extracted from Bidens pilosa. Three bioactivity isomers compounds, including methyl 3,4-dicaffeoyl quinate (1), methyl 3,5-dicaffeoyl quinate (2), and methyl 4,5-dicaffeoyl quinate (3) were successfully isolated by countercurrent chromatography (CCC) with two phase solvent system composed of chloroform-methanol-water-n-buthanol (4:3:2:0.5, v/v) elution. Their purities were 93.7%, 82.4%, and 91.8% determined by HPLC. HR-ESI-MS and NMR was used to confirm their structures. This is the first time the isolation of geometrical isomers of methyl dicaffeoyl quinates by CCC has been reported.

Notes

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