HPLC study of the inhibiting effect of phosphate and bicarbonate buffers on the leaching pattern of dental resin composites

ABSTRACT

A high-performance liquid chromatographic method was applied to evaluate the effect of bicarbonate (NaHCO₃) and phosphate (NaH₂PO₄, Na₂HPO₄) buffers on monomer leachability of dental resin composites. Evetric was the restorative resin composite that was investigated in current study. Forty disk-shaped resin composite specimens (n = 40) were immersed by means of a silk string in the following solutions: Distilled water (A), phosphate buffer (B), bicarbonate buffer (C), artificial saliva (D). After seven (7) and fourteen (14) days, the eluates were analyzed by means of High Performance Liquid Chromatography (HPLC). Subsequently, new specimens were fabricated and immersed in fresh solutions in which methanol 12% (v/v) was added. After seven and fourteen days of storage, the leaching of monomers was examined by means of HPLC, which was proved to be an effective tool to buffers demonstrate the inhibiting effect of phosphate and bicarbonate ions on the leaching pattern of dental resin composites.

GRAPHICAL ABSTRACT

KEYWORDS:

• Bisphenol A (BPA)
• bisphenol a glycerolate dimethacrylate (BIS-GMA)
• high performance liquid chromatography (HPLC)
• leaching inhibition
• monomers

Acknowledgments

All authors declare no financial and commercial conflict of interest.