Antibacterial activities of amorphous cefuroxime axetil ultrafine particles prepared by high gravity antisolvent precipitation (HGAP)

Abstract

In vitro and in vivo antibacterial activities on the Staphylococcus aureus and Escherichia coli of the amorphous cefuroxime axetil (CFA) ultrafine particles prepared by HGAP method were investigated in this paper. The conventional sprayed CFA particles were studied as the control group. XRD, SEM, BET tests were performed to investigate the morphology changes of the samples before and after sterile. The in vitro dissolution test, minimal inhibitory concentrations (MIC) and the in vivo experiment on mice were explored. The results demonstrated that: (i) The structure, morphology and amorphous form of the particles could be affected during steam sterile process; (ii) CFA particles with different morphologies showed varied antibacterial activities; and (iii) the in vitro and in vivo antibacterial activities of the ultrafine particles prepared by HGAP is markedly stronger than that of the conventional sprayed amorphous particles.

Keywords::

• Ultrafine particles
• in vivo
• antibacterial activity
• cefuroxime axetil
• amorphous

Acknowledgements

This work was financially supported by National Natural Science Foundation of China (Nos. 20806004 and 20821004) and the Talent Training Program of Beijing City (2007B022).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.