Characterization of a Corynebacterium Strain That Can Grow in Medium Containing up to 2 M Nitrate

ABSTRACT

A gram-positive bacterium, identified as Corynebacterium K37, was isolated from the waste effluent of a dairy farm. The bacterium thrived and expressed nitrate-reducing activity at nitrate concentrations of up to 2 M, and reduced nitrate concentration from 0.4 M to 11.4 mM and also from 0.4 M to 23.4 mM in aerobic and anaerobic fed-batch cultures, respectively. Cells of K37 were able to utilize a variety of carbon sources for nitrate reduction with little or no accumulation of nitrite. In aerobic cultures, the residual nitrite was minimal and it was completely reduced after prolonged incubation. Growth on acetate or pyruvate in anaerobic cultures resulted in lower nitrite reductase activities and concomitant higher residual nitrite concentrations than did growth on ethanol or glucose, suggesting that diminished electron availability was a factor in the accumulation of residual nitrite. The bacterium also survived in 2 M concentrations of NaCl, KCl, and CaCl₂. Corynebacterium sp. K37 may be useful in bioremediation of high nitrate pollution in contaminated soils and water.

KEYWORDS:

• aerobic
• anaerobic
• fed-batch cultures
• nitrite
• reductase

ACKNOWLEDGEMENTS

This research was supported by USDA-TSTAR award 2004–34135–14978.