Mercury resistance and plant growth promoting traits of endophytic bacteria isolated from mercury-contaminated soil

Abstract

Mercury (Hg) is a toxic trace element that can cause serious environmental problems. The main source of Hg contamination to soil across Indonesia is artisanal and small-scale gold mining (ASGM) and strategies to manage or remove Hg from soil are needed. This study isolated Hg-resistant endophytic bacteria from indigenous local grasses (Cynodon dactylon and Eleusine indica) growing in Hg-contaminated soils to identify potential inoculants that could increase plant growth and mercury uptake for phytoremediation. Grass samples were collected from an ASGM location on Lombok Island. The grass samples (both, root and shoot) were surface sterilized, aseptically mashed, and serially diluted with 0.9% sodium chloride (v/v) and an aliquot of the extracted bacteria was cultured on Nutrient Agar across a range of HgCl₂ concentration from 0 to 1000 mg/L. Thirteen bacterial isolates were tested for their ability to detoxify Hg, Hg bioaccumulation, indole acetic acid (IAA) production, ammonium production, and siderophore production. Five of thirteen isolates were Hg-resistant and survived on a medium containing 250 mg/L HgCl₂. One isolate from each of E. indica and C. dactylon showed complete Hg detoxification. The greatest Hg bioaccumulation was 85.04%. The highest expressed IAA and ammonium concentration was 13.17 mg/L and 2.58 mg/L respectively. Based on 16S rDNA sequence similarity, the two isolates with the greatest potential as inoculants (CD6 and EI6) were identified as Jeotgalicoccus huakuii (isolated from C. dactylon) and Bacillus amyloliquefaciens (isolated from E. indica) and could be used as inoculum for phytoremediation of Hg-contaminated soil in future studies.

Keywords:

• Endophytic diazotroph
• growth promoting endophytic
• Hg phytoremediation
• Hg-resistant bacteria
• indole acetic acid
• siderophore

Acknowledgments

The authors thank field and laboratory assistants in Indonesia, Ph.D fellows at Brawijaya and Massey University, and the Bonjeruk Farmers Association of Central Lombok, West Nusa Tenggara, Indonesia for their support.

Disclosure statement

There are no conflicting interests among authors.

Additional information

Funding

This research was funded by the Ministry of Education, Culture, Research and Technology of Indonesia through Master and PhD. Scholarship Programme (Beasiswa PMDSU).