Identification of Archaeal population in the granular sludge of an UASB reactor treating sewage at low temperatures

Abstract

Effect of low temperature on up-flow anaerobic sludge bed (UASB) reactor performance treating raw sewage was investigated in terms of the variations in methanogenic diversity using the 16S rRNA based Fluorescence In-Situ Hybridization (FISH) technique. The diversity of microorganisms present in the anaerobic granular sludge and the structure of the granules operated at 13°C have been investigated using FISH combined with CSLM (Confocal Scanning Laser Microscopy). According to FISH results, archaeal cells representing methanogens were found intensively dominant in the bottom sampling port of the UASB reactor and acetoclastic Methanosaeta was the abundant methanogen. Other methanogens such as Methanosarcina and Methanobacterium like species were also observed. The abundance of originally mesophilic Methanosaeta-related Archaea under low temperature at all sampling days revealed the microbial adaptation to psychrophilic conditions. This might be attributed to the enzymatic alterations in Methanosaeta cells originating from seed sludge, which were exposed to sub-mesophilic temperatures at start-up and then to psychrophilic conditions during gradual decreases of temperature. According to CSLM observation, even though the sludge retained in the reactor kept its granular form as a whole, the majority of the granules had a tendency to be partly broken and they lost their rigidity when raw sewage was fed following synthetic sewage. Besides, Methanosaeta related species prevailing in seed sludge have noticeably lost their long filamentous forms and deteriorated during raw sewage feeding. Members of the order Methanobacteriales constituted the major hydrogenothrophic methanogens present in the psychrophilic UASB reactor, whereas the other hydrogenothrophic methanogens—members of the order Methanococcales and Methanogenium relatives—were absent.

Keywords:

• CSLM
• FISH
• methanogenic diversity
• psychrophilic temperature
• sewage
• UASB reactor

Acknowledgement

The authors gratefully acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK) for the scholarship of NATO A-2 Science Fellowship Programme for 6 months in Germany. The Institute of Water Quality Control and Waste Management at Munich Technical University is acknowledged for providing the Confocal Scanning Laser Microscopy (CSLM). Besides, Research Fund of Istanbul Technical University (Project No: 1826) is also acknowledged for providing some part of the financial support.