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Journal of Environmental Science and Health, Part A
Toxic/Hazardous Substances and Environmental Engineering
Volume 53, 2018 - Issue 12
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Original Articles

Rapid identification of Streptomyces tetracycline producers by MALDI-TOF mass spectrometry

, , , , , , & show all
Pages 1083-1093 | Received 02 Mar 2018, Accepted 28 Apr 2018, Published online: 29 May 2018
 

ABSTRACT

The main objective of this study was using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for assembling of DSM (German Collection of Microorganisms) Streptomyces spectral database and identification of wild Streptomyces cultures, which were clustered by MALDI-TOF Biotyper OC software as well as for teracycline detection by observing of obtained spectra using flexAnalysis software. Production of tetracycline was confirmed by thin-layer chromatography. Presence of tetracycline mass spectrum was verified by several tetracycline producers (Streptomyces aureofaciens LMG 5968, S. aureofaciens 84/25, and S. aureofaciens BMK) and by pure tetracycline mass. Our results showed that it is possible to use MALDI-TOF MS for identification of tetracycline producers within Streptomyces genera by several easy steps. The purpose of this study was to establish cheap and quick detection of tetracycline producers.

Additional information

Funding

This work was supported by the Slovak Research and Development Agency under Contract no. APVV-16-0289 and Grant VEGA 1/0305/17.

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