Sustainable biological system for the removal of high strength ammoniacal nitrogen and organic pollutants in poultry waste processing industrial effluent

Figures & data

Table 1. Physicochemical parameters of the poultry waste processing industrial effluent before and after treatment

Parameters	Untreated effluent (mg/L)	After sequential treatment (mg/L)
pH	pH 8.48	pH 8.73
Carbohydrate	130 ± 3.9	128 ± 3.8
Protein	3,895 ± 16.5	366 ± 10.9
Lipid	178 ± 5.3	97 ± 2.9
Sulphate	184 ± 5.5	138 ± 4.1
Chloride	512 ± 10.3	432 ± 8.6
Ammonia	1,330 ± 13.9	120 ± 5.6
COD	46,500 ± 58	820 ± 8.64

Figure 1. (a) Screening of microbial consortia. (b) Phylogenetic tree of strain Bacillus albus.

Figure 2. Effect of treatment conditions: (a) time; (b) biomass concentration; amd (c) pH in stage I, aerobic treatment process

Table 2. Comparison of amino acids composition before and after treatment

Amino acids	Initial Effluent (w/w%)	Treated (aerobic) (w/w%)	Treated (anaerobic) (w/w%)
Aspartic acid	0.4805	0.3103	0.1338
Glutamic acid	0.085	0.054	0.2194
Asparagine	0.112	0.003	0.0548
Serine	0.128	0.093	0.0693
Glutamine	0.354	0.112	0.4596
Glycine	0.884	0.734	0.3956
Threonine	0.980	0.894	0.3198
Arginine	1.024	0.8485	0.6695
Alanine	0.342	0.1145	0.4039
Cysteine	0.8034	0.594	0.5954
Tyrosine	0.394	0.194	0.2298
Histidine	0.443	0.3045	0.5085
Valine	0.9532	0.7349	0.1935
Methionine	0.093	0.083	0.4293
Iso-leucine	0.545	0.339	0.2293
Phenylalanine	0.942	0.094	0.1094
Leucine	-	-	0.3048
Lysine	0.935	0.587	0.2983
Proline	-	-	0.2935
Tryptophan	0.1135	0.093	0.1935

Figure 3. (a) SDS PAGE of crude enzyme. (b) HPLC analysis. (c) FT-IR spectra of the stage-wise treated poultry waste processing industrial effluent

Figure 4. GC-MS analysis of (a) untreated effluent, (b) stage I, aerobic treatment, and (c) stage II, anaerobic treatment