Simultaneous pretreatment and saccharification process for fermentable sugars production from Casuarina equisetifolia biomass using transgenic Trichoderma atroviride

Figures & data

Figure 1. Effect of incubation time on the production of enzyme cocktail and fermentable sugars. All the experiments were performed in triplicates and the results were represented as mean average with standard deviation

Table 1. Effect of temperature and pH on enzyme cocktail production of transgenic Trichoderma atroviride.

S. No	Temperature (ºC)	pH	Laccase (U/kg)	Cellulase (U/kg)	Xylanase (U/kg)	Protein (mg/ml)	Fermentable sugars (g/kg)
1	25	4	62.31 ± 1.24	725.36 ± 2.01	825.36 ± 2.35	1.88 ± 0.42	106.35 ± 2.21
2		5	75.25 ± 1.41	625.36 ± 1.92	725.36 ± 2.72	2.21 ± 0.48	112.36 ± 2.03
3		6	73.36 ± 0.85	612.36 ± 2.14	714.36 ± 1.65	2.32 ± 0.55	100.23 ± 1.92
4	30	4	85.36 ± 0.92	825.36 ± 1.04	924.36 ± 1.74	2.74 ± 0.53	193.34 ± 2.01
5		5	173.36 ± 1.72	1145.9 ± 1.33	1025.36 ± 2.65	3.49 ± 0.24	221.12 ± 2.35
6		6	151.8 ± 1.34	727.4 ± 2.24	758.8 ± 2.62	2.53 ± 0.31	201.35 ± 1.65
7	35	4	29.526 ± 0.24	325.63 ± 2.45	797.6 ± 1.84	2.71 ± 0.48	102.21 ± 1.85
8		5	14.364 ± 1.32	557.9 ± 1.95	665.9 ± 1.63	2.53 ± 0.16	142.34 ± 1.65
9		6	23.883 ± 0.54	547.9 ± 1.85	722.5 ± 2.05	2.36 ± 0.14	124.42 ± 1.24
10	40	4	23.36 ± 0.75	325.36 ± 2.15	453.6 ± 2.24	0.23 ± 0.34	65.36 ± 2.01
11		5	12.36 ± 0.92	214.36 ± 1.15	205.31 ± 2.36	0.75 ± 0.25	84.25 ± 1.54
12		6	10.36 ± 1.15	195.36 ± 1.25	185.36 ± 1.35	0.52 ± 0.13	78.25 ± 1.25

All the experiments were performed in triplicates and the results were represented as mean average with standard deviation.

Figure 2. Effect of (a) inoculum age and (b) inoculum size on the production of enzyme cocktail and fermentable sugars. All the experiments were performed in triplicates and the results were represented as mean average with standard deviation

Figure 3. (a) Saccharification kinetics and (b) biomass load of Casuarina biomass for simultaneous pre-treatment and saccharification (SPS) process. All the experiments were performed in triplicates and the results were represented as mean average with standard deviation

Figure 4. Comparative toxicity analysis of SPS with respective conventional acid treatment and alkaline treatment methods on Casuarina biomass. All the experiments were performed in triplicates and the results were represented as mean average with standard deviation