Differential response to hormesis by laboratory evolved short-lived and long-lived cytoraces of nasuta-albomicans complex of Drosophila

Figures & data

Figure 1. Mean lifespan ± S.E of control, RMHS, BuA and TSA treated males (a) and females (b) of all the eight members of nasuta-albomicans complex of Drosophila under study.

Figure 2. Survivorship (lx) of control and RMHS treated males (a) and females (b) of all the members of nasuta-albomicans complex of Drosophila. All the eight races with initial C indicate control and without C is RMHS treated.

Figure 3. Survivorship (lx) of control and BuA treated males (a) and females (b) of all the members of nasuta-albomicans complex of Drosophila. All the eight races with initial C indicate control and without C is BuA treated.

Figure 4. Survivorship (lx) of control and TSA treated males (a) and females (b) of all the members of nasuta-albomicans complex of Drosophila. All the eight races with initial C indicate control and without C is TSA treated.

Table I. Comparison of lifespan of control flies with repeated mild heat shock (RMHS)-treated laboratory-evolved flies of Drosophila by log-rank test, where n = 100 and df = 1

Races	Sex	Log-rank test
		χ^2	p-value	Hazard ratio	95% Confidence interval
N	♂	0.4078	0.5231	1.1019	0.8181–1.4842
	♀	12.7554	0.0004	1.7726	1.2947–2.4268
A	♂	14.7916	0.0001	1.8318	1.3456–2.4938
	♀	8.5009	0.0035	1.5943	1.1652–2.1814
SL-1	♂	17.9523	<0.0001	1.9597	1.4356–2.6751
	♀	13.2398	0.0003	1.7687	1.3009–2.4048
SL-2	♂	42.8609	<0.0001	2.9971	2.1576–4.1630
	♀	37.2167	<0.0001	2.7797	2.0015–3.8605
LL-1	♂	18.5696	<0.0001	2.0359	1.4734–2.8132
	♀	5.4988	0.0190	1.4371	1.0613–1.9460
LL-2	♂	12.8502	0.0003	1.7676	1.2946–2.4135
	♀	0.7553	0.3848	1.1427	0.8458–1.5439
LL-3	♂	34.0633	<0.0001	2.6239	1.8978–3.6277
	♀	19.0804	<0.0001	2.0073	1.4683–2.7440
LL-4	♂	22.0811	<0.0001	2.1833	1.5764–3.0239
	♀	37.2723	<0.0001	2.8127	2.0181–3.9202

Table II. Distribution of percent survivability in control males and females of all the eight members of the nasuta-albomican s complex of Drosophila.

Week	NM	NF	AM	AF	SL-1M	SL-1F	SL-2M	SL-2F	LL-1M	LL-1F	LL-2M	LL-2F	LL-3M	LL-3F	LL-4M	LL-4F
3	100
4	99				100	100
5	94	100	100		98	99	100	100
6	58	96	81	100	69	83	87	95
7	42	79	57	97	38	69	57	65
8	28	50	28	84	8	45	22	34
9	2	10	5	29	2	11	4	8
10	0	0	0	2	0	1	0	0							100
11				0		0			100				100	100	99
12									86		100		84	99	94
13									36		84		45	98	54	100
14									0	100	49	100	11	80	8	94
15										96	13	97	1	45	0	69
16										68	0	84	0	10		28
17										30		52		0		0
18										7		31
19										0		4
20												0
NM, D. n .nasuta male; NF, D. n. nasuta female; AM, D. n. albomicans male; AF, D. n. albomicans female; SL-1M, short-lived cytorace 3 male; SL-1F, short-lived cytorace 3 female; SL-2M, short-lived cytorace 15 male; SL-2F, short-lived cytorace 15 female; LL-1M, long-lived cytorace 2 male; LL-1F, long-lived cytorace 2 female; LL-2M, long-lived cytorace 9 male; LL-2F, long-lived cytorace 9 female; LL-3M, long-lived cytorace 11 male; LL-3F, long-lived cytorace 11 female; LL-4M, long-lived cytorace 16 male; LL-4F, long-lived cytorace 16 female.

Table III. Distribution of percent survivability in repeated mild heat shock (RMHS)-treated males and females of all eight members of the nasuta-albomicans complex of Drosophila.

Week	NM	NF	AM	AF	SL-1M	SL-1F	SL-2M	SL-2F	LL-1M	LL-1F	LL-2M	LL-2F	LL-3M	LL-3F	LL-4M	LL-4F
5	100				100
6	83	100	100	100	97	100	100	100
7	53	92	75	97	60	89	88	89
8	27	56	42	84	29	56	56	63
9	2	28	15	56	5	28	23	34
10	0	1	2	2	0	6	2	8
11		0	0	0		0	0	1	100				100		100
12								0	84				97	100	95
13									54		100		66	96	64	100
14									13	100	67		35	90	29	97
15									0	96	29	100	10	63	8	81
16										81	0	92	0	35	0	56
17										37		62		6		23
18										12		26		0		6
19										1		2				0
20										0		0

Table IV. Comparison of lifespan of control flies with sodium butyrate (BuA)-treated laboratory-evolved flies of Drosophila by log-rank test where n= 100 and df = 1

Races	Sex	Log-rank test
		χ^2	p-value	Hazard ratio	95% Confidence interval
N	♂	8.0839	0.0045	1.5504	1.1459–2.0975
	♀	24.6665	<0.0001	2.2615	1.6388–3.1207
A	♂	59.4038	<0.0001	3.8254	2.7196–5.3808
	♀	23.1428	<0.0001	2.2057	1.5980–3.0445
SL-1	♂	37.375	<0.0001	2.761	1.9937–3.8236
	♀	55.4685	<0.0001	3.6011	2.5704–5.0451
SL-2	♂	150.1833	<0.0001	11.8152	7.9601–17.5373
	♀	150.8112	<0.0001	11.9557	8.0462–17.7648
LL-1	♂	3.713	0.0540	1.3603	0.9947–1.8602
	♀	1.5249	0.2169	1.2076	0.8951–1.6292
LL-2	♂	1.1278	0.2882	1.1786	0.8703–1.5962
	♀	0.1184	0.7307	1.0538	0.7818–1.4205
LL-3	♂	44.7289	<0.0001	3.0982	2.2243–4.3156
	♀	28.6316	<0.0001	2.3869	1.7355–3.2827
LL-4	♂	54.5737	<0.0001	3.6929	2.6112–5.2228
	♀	11.6798	0.0006	1.7241	1.2615–2.3563

Table V. Distribution of percent survivability in sodium butyrate (BuA)-treated males and females of all eight members of the nasuta-albomicans complex of Drosophila.

Week	NM	NF	AM	AF	SL-1M	SL-1F	SL-2M	SL-2F	LL-1M	LL-1F	LL-2M	LL-2F	LL-3M	LL-3F	LL-4M	LL-4F
5	100
6	88	100	100	100	100
7	63	95	88	97	71	100	100	100
8	37	67	63	90	40	80	92	97
9	6	35	37	64	8	48	58	69
10	0	4	8	8	0	17	28	39
11		0	0	0		0	3	11	100
12							0	0	84		100		100		100
13									44		92		78	100	99	100
14									1	100	55		45	97	76	97
15									0	96	18	100	12	69	45	71
16										75	0	89	0	39	13	43
17										29		53		11	0	11
18										11		28		0		0
19										1		3
20										0		0

Table VI. Comparison of lifespan of control flies with Trichostatin A (TSA)-treated laboratory-evolved flies of Drosophila by log-rank test where n=100 and df=1

Races	Sex	Log-rank test
		χ^2	p-value	Hazard ratio	95% Confidence interval
N	♂	44.859	<0.0001	3.0802	2.2162–4.2811
	♀	37.6303	<0.0001	2.8205	2.0251–3.9284
A	♂	108.7142	<0.0001	7.1813	4.9574–10.4028
	♀	43.8576	<0.0001	3.135	2.2355–4.3964
SL-1	♂	78.4737	<0.0001	4.8962	3.4453–6.9581
	♀	178.1724	<0.0001	16.4037	10.8778–24.7365
SL-2	♂	240.1417	<0.0001	34.3736	21.9747–53.7684
	♀	240.3992	<0.0001	34.2252	21.8970–53.4941
LL-1	♂	3.8191	0.0507	1.3672	0.9991–1.8708
	♀	3.6249	0.0569	1.3444	0.9913–1.8231
LL-2	♂	3.7618	0.0524	1.3544	0.9968–1.8402
	♀	1.2885	0.2563	1.1889	0.8819–1.6027
LL-3	♂	163.5949	<0.0001	13.8575	9.2626–20.7318
	♀	155.8608	<0.0001	12.5919	8.4602–18.7413
LL-4	♂	63.5638	<0.0001	4.2772	2.9922–6.1140
	♀	200.1342	<0.0001	21.4964	14.0531–32.8821

Table VII. Distribution of percent survivability in Trichostatin A (TSA)-treated males and females of all eight members of the nasuta-albomicans complex of Drosophila.

Week	NM	NF	AM	AF	SL-1M	SL-1F	SL-2M	SL-2F	LL-1M	LL-1F	LL-2M	LL-2F	LL-3M	LL-3F	LL-4M	LL-4F
5	100
6	99	100			100
7	77	97	100	100	87
8	53	73	78	92	55	100
9	29	41	53	64	22	86	100
10	3	6	29	34	0	56	94	100
11	0	0	1	2		26	68	85	100						100
12			0	0		0	34	57	90		100		100		96
13							11	30	43		97		97		68
14							0	1	1		57		53		55
15								0	0	100	22	100	34	100	40	100
16										77	0	92	18	81	25	99
17										34		61	0	49	1	75
18										9		30		22	0	48
19										0		2		0		17
20												0				0