Leukocyte differential counts and morphology from twelve European lizards

Figures & data

Figure 1. Heterophils, eosinophils and basophils of cells of the Laceta bilineata, L. viridis, L. trilineata, L. oxycephala, Timon lepidus and Zootoca vivipara (May–Grünwald/Giemsa stain). Scale bar: 10 μm.

Figure 2. Heterophils, eosinophils and basophils of cells of the Podarcis tiliguerta, P. muralis, P. melisellensis, P. sicula, P. bocagei, and Algyroides nigropunctatus (May–Grünwald/Giemsa stain). Scale bar: 10 μm.

Figure 3. The fourth granulocyte type (a–h), and an erythrocyte infected by haemogregarine spp. (i) (May–Grünwald/Giemsa stain); (a) Podarcis tiliguerta, (b) P. muralis, (c) P. melisellensis , (d) P. sicula, (e) Algyroides nigropunctatus, (f) Lacerta bilineata, (g) L. trilineata, and (h) L. oxycephala. Scale bar: 10 μm.

Table I. WBC, differential leukocyte count and thrombocyte count from the blood of P. muralis

	Males (N = 71)		Females (N = 10)		Overall (N = 81)
Blood cell type	Mean ± SE	Range	Mean ± SE	Range	Mean ± SE	Range
WBC (N/10,000 erythrocytes)	222 ± 11	100–635	117 ± 10	58–168	209 ± 11	100–635
Heterophils (%)	15.5 ± 1.2	0.0–43.6	14.8 ± 2.7	1.3–28.5	15.5 ± 1.1	0.0–43.6
Eosinophils (%)	13.2 ± 1.0	0.0–41.6	16.0 ± 4.5	4.3–46.1	13.5 ± 1.0	0.0–46.1
Basophils (%)	6.7 ± 0.6	0.6–23.5	12.0 ± 1.8	4.8–21.4	7.4 ± 0.6	0.6–23.5
Type IV (%)	0.2 ± 0.1	0.0–1.3	0.0		0.2 ± 0.1	0.0–1.3
Monocytes (%)	0.4 ± 0.1	0.0–2.0	0.7 ± 0.5	0.0–4.9	0.4 ± 0.1	0.0–4.9
Lymphocytes (%)	63.9 ± 2.1	6.7–90.5*	56.6 ± 5.1	20.6–71.7	63.0 ± 2.0	6.7–90.5*
Thrombocytes (N/10,000 erythrocytes)	84 ± 15	0–770	87 ± 44	0–383	85 ± 14	0–770
*The low value of the inferior limit was due to a male showing high values of both heterophils and eosinophils, but without any sign of abnormality.

Table II. WBC, differential leukocyte count and thrombocyte count from the blood of A. nigropunctatus

	Males (N = 5)		Females (N = 2)		Overall (N = 7)
Blood cell type	Mean ± SE	Range	Mean ± SE	Range	Mean ± SE	Range
WBC (N/10,000 erythrocytes)	227 ± 25	155–305	278 ± 71	207–349	241 ± 25	155–349
Heterophils (%)	17.4 ± 2.5	11.9–25.8	11.5 ± 3.6	7.9–15.0	15.7 ± 2.2	7.9–25.8
Eosinophils (%)	8.3 ± 1.6	3.8–13.8	6.0 ± 0.7	5.2–6.7	7.7 ± 1.2	3.8–13.8
Basophils (%)	9.5 ± 1.7	4.4–14.7	7.4 ± 3.7	3.7–11.1	8.9 ± 1.5	3.7–14.7
Type IV (%)	0.2 ± 0.2	0.0–1.1	0.3 ± 0.3	0.0–0.7	0.3 ± 0.2	0.0–1.1
Monocytes (%)	0.6 ± 0.2	0.0–1.3	0.0		0.4 ± 0.2	0.0–1.3
Lymphocytes (%)	63.9 ± 2.8	56.4–73.8	74.8 ± 6.9	68.0–81.7	67.0 ± 3.2	56.4–81.7
Thrombocytes (N/10,000 erythrocytes)	73 ± 17	25–112	65 ± 33	33–98	70 ± 14	25–112

Table III. WBC, differential leukocyte count and thrombocyte count from the blood of P. bocagei

	Males (N = 6)		Females (N = 5)		Overall (N = 11)
Blood cell type	Mean ± SE	Range	Mean ± SE	Range	Mean ± SE	Range
WBC (N/10,000 erythrocytes)	304 ± 21	243–369	218 ± 31	138–328	265 ± 22	138–369
Heterophils (%)	6.9 ± 1.4	3.0–11.4	9.1 ± 2.9	0.0–16.9	7.9 ± 1.5	0.0–16.9
Eosinophils (%)	10.6 ± 1.6	7.1–15.2	15.2 ± 3.9	4.3–24.5	12.7 ± 1.9	4.3–24.5
Basophils (%)	13.5 ± 1.7	6.3–20.7	13.7 ± 3.3	7.3–25.3	13.6 ± 1.8	6.3–25.3
Type IV (%)	1.7 ± 0.2	0.4–3.6	0.9 ± 0.3	0.6–1.9	1.3 ± 0.4	0.4–3.6
Monocytes (%)	0.7 ± 0.2	0.5–1.2	0.6 ± 0.1	0.0–1.2	0.7 ± 0.1	0.0–1.2
Lymphocytes (%)	66.6 ± 2.8	58.8–73.8	60.5 ± 7.2	38.6–81.7	63.9 ± 3.8	38.6–81.7
Thrombocytes (N/10,000 erythrocytes)	115 ± 21	44–183	98 ± 17	38–133	108 ± 13	38–183

Table IV. WBC, differential leukocyte count and thrombocyte count from the blood of P. melisellensis

	Males (N = 7)		Females (N = 5)		Overall (N = 11)
Blood cell type	Mean ± SE	Range	Mean ± SE	Range	Mean ± SE	Range
WBC (N/10,000 erythrocytes)	145 ± 16	104–210	207 ± 45	129–355	171 ± 22	104–355
Heterophils (%)	8.8 ± 1.4	2.6–13.5	15.1 ± 4.5	4.2–27.2	11.4 ± 2.2	2.6–27.2
Eosinophils (%)	9.3 ± 1.7	3.9–16.0	10.3 ± 2.8	3.8–19.7	9.7 ± 1.4	3.8–19.7
Basophils (%)	4.5 ± 1.2	1.3–9.1	5.9 ± 1.9	0.6–11.9	5.1 ± 1.0	0.6–11.9
Type IV (%)	0.0		0.3 ± 0.3	0.0–1.4	0.1 ± 0.1	0.0–1.4
Monocytes (%)	0.0		0.2 ± 0.1	0.0–0.7	0.1 ± 0.1	0.0–0.7
Lymphocytes (%)	77.4 ± 2.7	69.2–90.1	68.2 ± 7.2	47.7–85.6	73.6 ± 3.5	47.7–90.1
Thrombocytes (N/10,000 erythrocytes)	11 ± 8	0–59	13 ± 11	0–56	12 ± 6	0–59

Table V. WBC, differential leukocyte count and thrombocyte count from the blood of P. sicula

	Males (N = 5)		Females (N = 5)		Overall (N = 10)
Blood cell type	Mean ± SE	Range	Mean ± SE	Range	Mean ± SE	Range
WBC (N/10,000 erythrocytes)	189 ± 36	113–302	220 ± 15	174–253	205 ± 19	113–302
Heterophils (%)	12.7 ± 5.6	6.2–35.0	5.4 ± 0.7	4.1–7.9	9.0 ± 2.9	4.1–35.0
Eosinophils (%)	9.9 ± 3.3	1.2–17.3	8.7 ± 1.1	4.4–10.5	9.3 ± 1.7	1.2–17.3
Basophils (%)	11.1 ± 4.5	3.7–28.3	13.5 ± 2.6	5.9–21.9	12.3 ± 2.5	3.7–28.3
Type IV (%)	0.2 ± 0.2	0.0–1.2	0.8 ± 0.5	0.0–2.0	0.5 ± 0.3	0.0–2.0
Monocytes (%)	1.5 ± 0.3	0.6–2.5	2.7 ± 1.1	0.0–5.7	2.1 ± 0.6	0.0–5.7
Lymphocytes (%)	64.5 ± 7.7	46.1–81.4	69.0 ± 4.4	59.9–80.5	66.8 ± 4.2	46.1–81.4
Thrombocytes (N/10,000 erythrocytes)	135 ± 74	0–334	101 ± 37	7–224	118 ± 40	0–334

Table VI. WBC, differential leukocyte count and thrombocyte count from the blood of P. tiliguerta

	Males (N = 4)		Females (N = 4)		Overall (N = 8)
Blood cell type	Mean ± SE	Range	Mean ± SE	Range	Mean ± SE	Range
WBC (N/10,000 erythrocytes)	277 ± 84	55–436	283 ± 45	193–397	280 ± 44	55–436
Heterophils (%)	5.6 ± 1.6	1.4–8.3	8.7 ± 2.2	5.3–15.2	7.1 ± 1.4	1.4–15.2
Eosinophils (%)	16.8 ± 3.8	10.1–26.2	16.8 ± 5.4	6.9–28.5	16.8 ± 3.1	6.9–28.5
Basophils (%)	10.0 ± 2.6	6.5–17.7	13.7 ± 1.6	11.8–18.5	11.8 ± 1.6	6.5–18.5
Type IV (%)	3.9 ± 1.0	1.0–5.5	3.0 ± 1.5	0.9–7.3	3.4 ± 0.8	0.9–7.3
Monocytes (%)	0.3 ± 0.1	0.0–0.6	0.7 ± 0.4	0.0–2.0	0.5 ± 0.2	0.0–2.0
Lymphocytes (%)	63.5 ± 7.6	43.3–77.3	57.1 ± 10.4	28.5–73.9	60.3 ± 6.1	28.5–77.3
Thrombocytes (N/10,000 erythrocytes)	87 ± 9	62–102	85 ± 12	68–120	86 ± 7	62–120

Figure 4. Haemogregarine loads (N/10,000 erythrocytes) in six European lacertid lizards. Numbers above boxes indicate sample sizes.