Preliminary data on population dynamics and genetics of Alitta succinea (Polychaeta: Nereididae) from the Romanian coast of the Black Sea

Figures & data

Figure 1. Location of the sampling sites of Alitta succinea, Romanian coast of the Black Sea.

Figure 2. Relationship between the length L3 and the fresh weight (a) and between the length L3 and the dry weight (b) of Alitta succinea from station C, Romanian coast of the Black Sea (N = 100 individuals).

Figure 3. Temporal variation of density of the population of Alitta succinea from station C, Romanian coast of the Black Sea (mean values ± SD).

Figure 4. Temporal variations of the biomass (wet weight) of the population of Alitta succinea from station C, Romanian coast of the Black Sea (mean values ± SD).

Table I. Secondary production of Alitta succinea from the Danube-Black Sea Canal, Romanian coast of the Black Sea (ΔW, weight variation of the cohort between t ₁ and t ₂;, mean number of individuals of the cohort between t ₁ and t ₂)

Month	Abundance N		Biomass W (g)	ΔW(g)	P (g m^−2)
June 2007	100	50	0.072	0.072	3.600
July 2007	93	96.5	0.046	–0.026	–2.510
August 2007	51	72	0.030	–0.016	–1.150
Total C2					–0.060
September 2007	79	39.5	0.005	0.005	0.197
October 2007	137	108	0.018	0.013	1.404
November 2007	196	166.5	0.020	0.002	0.333
December 2007	109	152.5	0.031	0.011	1.677
January 2008	34	71.5	0.030	–0.001	–0.071
February 2008	44	39	0.058	0.028	1.092
March 2008	44	44	0.054	–0.004	–0.176
April 2008	51	47.5	0.137	0.083	3.942
May 2008	68	59.5	0.092	–0.045	–2.677
Total C3					5.721
TOTAL					5.661

Figure 5. Size frequency histograms of Alitta succinea from station C, Romanian coast of the Black Sea.

Figure 6. Growth curves of the cohorts of Alitta succinea from station C, Romanian coast of the Black Sea.

Table II. Different tested enzymatic systems: enzymatic activity, resolution and patterns variability (+ low, ++ mean, +++ strong) for Alitta succinea

Enzymes	Enzyme activity	Resolution	Variability
EST	++	++	+++
IDH	++	++	-
MDH	++	++	-
LDH	++	+	+
LAP	+	+	+++
PGM	+++	++	++

Table III. Genotype, allele frequencies and observed (Ho) and expected (He) heterozygosity estimates for Alitta succinea (site A: Agigea and site C: Danube–Black Sea Canal)

	Genotypes							Allele frequencies
	N	aa	bb	cc	ab	bc	ac	a	b	c	He	Ho	Fis
Site C	23	0	16	0	5	2	0	0.109	0.848	0.044	0.268	0.304	–0.134
Site A	25	0	16	0	7	2	0	0.140	0.820	0.040	0.306	0.360	–0.184

Figure 7. PGM enzymatic patterns and corresponding genotypes of Alitta succinea, Romanian coast of the Black Sea.

Figure 8. EST variable patterns in the anodic zone of the gel of Alitta succinea, Romanian coast of the Black Sea.

Table IV. Genetic parameters for Alitta succinea (site A: Agigea and site C: Danube–Black Sea Canal) based on 29 RAPD loci analysis (h, Nei's gene diversity; I, Shannon's information index; Nm, estimate of gene flow from Gst (Nm = 0.5 (1 – Gst)/ Gst))

Groups	Polymorphic bands	PPB (%)	h	I	Gst	Nm
Site A	24	82.76	0.1873	0.3066
Site C	23	79.31	0.2099	0.3314
A+C	29	100.00	0.2172	0.3523	0.3076	1.12

Figure 9. Electrophoresis agarose gel profiles of RAPD products amplified with RAPD8 primer from DNA of 20 Alitta succinea individuals sampled at Agigea (population A; individuals 1 to 10) and in the Danube–Black Sea Canal (population C; individuals 11 to 20), Romanian coast of the Black Sea.

Figure 10. UPGMA dendrogram based on Nei's genetic distance estimated between 20 Alitta succinea individuals sampled at Agigea (population A; individuals 1 to 10) and in the Danube–Black Sea Canal (population C; individuals 11 to 20), Romanian coast of the Black Sea.

Table V. Main characteristics of the populations of Alitta succinea in the world ordered by latitude

Localities	Authors	Density (ind. m^−²)	Biomass(g m^−²)
Isefjord (Kattegat Strait)	Rasmussen 1973	∼600	–
Kiel Fjord (Baltic Sea)	Neuhoff 1979	35–171	24.5–119.7 (FW)^a
Sea of Azov	Stark 1959	–	0–40.41 (FW)
Sukhoi Lake (NW Black Sea)	Losovskaya 1977	670^b	2.4 (FW)^b
Romanian coast (Black Sea)	Ţigănuş 1986	5587–7487^b	–
	Ţigănuş 1988	0–8120^b	–
	Ţigănuş 1992	129–4000	–
	Surugiu 2000	187–5568	4.27–131.50 (FW)
	Surugiu 2003	44–5617	0.10–46.49 (FW)
	Present study	125–1633	3.90–63.78 (FW)1.15–8.79 (DW)
Izmir Bay (E Mediterranean)	Çinar et al. 2008	175–2050	–
Salton Sea (California)	Detwiler et al. 2002	0–81,153	0–32.19 (FW)
a, values derived from biomass expressed in kJ m^−2; b, mean annual values.

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